2009
c-Myb prevents apoptosis of colon carcinoma cells in Cox-2-independent manner
KNOPFOVÁ, Lucia; Kristina NEŠPOROVÁ; Petr BENEŠ a Jan ŠMARDAZákladní údaje
Originální název
c-Myb prevents apoptosis of colon carcinoma cells in Cox-2-independent manner
Název česky
c-Myb snižuje frekvenci apoptózy buněk střevního karcinomu nezávisle na aktivitě cyklooxygenázy-2
Název anglicky
c-Myb prevents apoptosis of colon carcinoma cells in Cox-2-independent manner
Autoři
Vydání
Athens Greece, International Journal of Molecular Medicine, s. 12-13, 2009
Nakladatel
D.A. Spandidos
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.980
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/09:00028620
Organizační jednotka
Přírodovědecká fakulta
ISSN
UT WoS
Klíčová slova česky
apoptóza střevní karcinom c-Myb cyklooxygenáza-2 cisplatina TRAIL
Klíčová slova anglicky
apoptosis colon carcinoma c-Myb cyclooxygenase-2 cisplatin TRAIL
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 9. 7. 2010 10:23, prof. RNDr. Jan Šmarda, CSc.
V originále
Development of the colon tumors is associated with mutations in various protooncogenes and tumor suppressor genes. c-myb codes for transcription factor that is involved in colorectal cancerogenesis. c-myb expression is frequently up-regulated in colon carcinomas compared to non-malignant colonic mucosa. The set of the Myb-targeted genes includes the cox-2 gene coding for cyclooxygenase 2. The aim of this study is to clarify the role of c-Myb and Cox-2 cooperation in regulation of apoptosis of colon carcinoma cell lines HCT116 and HT-29. We prepared HCT116 cells overexpressing c-Myb and HT-29 cells with silenced c-myb expression and confirmed that up- and down-regulation of c-Myb correlate with expression of cox-2. Next, we studied the role of c-Myb/Cox-2 in regulation of apoptosis in response to cis-platin and TRAIL. We showed that overexpression of c-myb stimulates resistance, while silencing of c-myb stimulates sensitivity of the colon carcinoma cells to both cis-platin and TRAIL.
Anglicky
Development of the colon tumors is associated with mutations in various protooncogenes and tumor suppressor genes. c-myb codes for transcription factor that is involved in colorectal cancerogenesis. c-myb expression is frequently up-regulated in colon carcinomas compared to non-malignant colonic mucosa. The set of the Myb-targeted genes includes the cox-2 gene coding for cyclooxygenase 2. The aim of this study is to clarify the role of c-Myb and Cox-2 cooperation in regulation of apoptosis of colon carcinoma cell lines HCT116 and HT-29. We prepared HCT116 cells overexpressing c-Myb and HT-29 cells with silenced c-myb expression and confirmed that up- and down-regulation of c-Myb correlate with expression of cox-2. Next, we studied the role of c-Myb/Cox-2 in regulation of apoptosis in response to cis-platin and TRAIL. We showed that overexpression of c-myb stimulates resistance, while silencing of c-myb stimulates sensitivity of the colon carcinoma cells to both cis-platin and TRAIL.
Návaznosti
| GA301/09/1115, projekt VaV |
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| GD204/08/H054, projekt VaV |
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| IAA501630801, projekt VaV |
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| MSM0021622415, záměr |
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