pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of
Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs
Upstream of the c-kit Transcription Initiation Site

J 2009

pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs Upstream of the c-kit Transcription Initiation Site

BUČEK, Pavel; Joaquim JAUMOT; Anna AVINO; Ramon ERITJA; Raimundo GARGALLO et. al.

Základní údaje

Originální název

pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs Upstream of the c-kit Transcription Initiation Site

Název česky

pH-modulace rovnováh Watson-Crickova duplexu a quadruplexu sekvencí bohatých na guanin a cytosin 140 bp za počátečním místem transkripce genu c-kit

Autoři

BUČEK, Pavel; Joaquim JAUMOT; Anna AVINO; Ramon ERITJA a Raimundo GARGALLO

Vydání

Chemistry - A European Journal, Weinheim, WILEY-VCH Verlag, 2009, 0947-6539

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Wiley InterScience Article

Impakt faktor

Impact factor: 5.382

Kód RIV

RIV/00216224:14310/09:00037215

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000272509500018

Klíčová slova česky

chemometrie struktur DNA G-kvadruplexy multivariátní analýza

Klíčová slova anglicky

chemometrics DNA structures G-quadruplexes multivariate analysis

Štítky

G_quadruplex, Multivariate analysis, non-canonical DNA spectroscopy chemometrics, Oligonucleotides, spectroscopy

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 8. 2011 08:20, Mgr. Pavel Buček, Ph.D.

Anotace

ORIG CZ

V originále

Guanine-rich regions of DNA are sequences capable of forming G-quadruplex structures. The formation of a G-quadruplex structure in a region 140 base pairs (bp) upstream of the c-kit transcription initiation site was recently proposed (Fernando et al., Biochemistry, 2006, 45, 7854). In the present study, the acid-base equilibria and the thermally induced unfolding of the structures formed by a guanine-rich region and by its complementary cytosine-rich strand in c-kit were studied by means of circular dichroism and molecular absorption spectroscopies. In addition, competition between the Watson-Crick duplex and the isolated structures was studied as a function of pH value and temperature. Multivariate data analysis methods based on both hard and soft modeling were used to allow accurate quantification of the various acid-base species present in the mixtures. Results showed that the G-quadruplex and i-motif coexist with the Watson-Crick duplex over the pH range from 3.0 to 6.5, approximately. Under the experimental conditions tested in this study. At pH 7.0, the duplex is practically the only species present.

Česky

Struktura na pozici 140bp a dále od počátku transkripce genu c-kit tvoří G-kvadruplex. Studium rovnováh mezi G-kvadruplexem a dalšími strukturami oligonukleotidu pomocí spektrálních metod. Experimentální data vyhodnocena pomocí soft- i hard-modelingových technik pokročilé chemometrie. Modelace chování systému za různých podmínek, posun rovnováh za různého pH.

Citovat

BUČEK, Pavel; Joaquim JAUMOT; Anna AVINO; Ramon ERITJA a Raimundo GARGALLO. pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs Upstream of the c-kit Transcription Initiation Site. Chemistry - A European Journal. Weinheim: WILEY-VCH Verlag, 2009, roč. 15/2009, č. 46, s. 12663-12671, 8 s. ISSN 0947-6539.

@article{855569,
   author = {Buček, Pavel and Jaumot, Joaquim and Avino, Anna and Eritja, Ramon and Gargallo, Raimundo},
   article_location = {Weinheim},
   article_number = {46},
   keywords = {chemometrics DNA structures G-quadruplexes multivariate analysis},
   language = {eng},
   issn = {0947-6539},
   journal = {Chemistry - A European Journal},
   title = {pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs Upstream of the c-kit Transcription Initiation Site},
   url = {http://www3.interscience.wiley.com/cgi-bin/fulltext/122658932/HTMLSTART},
   volume = {15/2009},
   year = {2009}
}

TY  - JOUR
ID  - 855569
AU  - Buček, Pavel - Jaumot, Joaquim - Avino, Anna - Eritja, Ramon - Gargallo, Raimundo
PY  - 2009
TI  - pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs Upstream of the c-kit Transcription Initiation Site
JF  - Chemistry - A European Journal
VL  - 15/2009
IS  - 46
SP  - 12663-12671
EP  - 12663-12671
PB  - WILEY-VCH Verlag
SN  - 09476539
KW  - chemometrics DNA structures G-quadruplexes multivariate analysis
UR  - http://www3.interscience.wiley.com/cgi-bin/fulltext/122658932/HTMLSTART
N2  - Guanine-rich regions of DNA are sequences capable of forming G-quadruplex structures. The formation of a G-quadruplex structure in a region 140 base pairs (bp) upstream of the c-kit transcription initiation site was recently proposed (Fernando et al., Biochemistry, 2006, 45, 7854). In the present study, the acid-base equilibria and the thermally induced unfolding of the structures formed by a guanine-rich region and by its complementary cytosine-rich strand in c-kit were studied by means of circular dichroism and molecular absorption spectroscopies. In addition, competition between the Watson-Crick duplex and the isolated structures was studied as a function of pH value and temperature. Multivariate data analysis methods based on both hard and soft modeling were used to allow accurate quantification of the various acid-base species present in the mixtures. Results showed that the G-quadruplex and i-motif coexist with the Watson-Crick duplex over the pH range from 3.0 to 6.5, approximately. Under the experimental conditions tested in this study. At pH 7.0, the duplex is practically the only species present.
ER  -

BUČEK, Pavel; Joaquim JAUMOT; Anna AVINO; Ramon ERITJA a Raimundo GARGALLO. pH-Modulated Watson-Crick Duplex-Quadruplex Equilibria of Guanine-Rich and Cytosine-Rich DNA Sequences 140 Base Pairs Upstream of the c-kit Transcription Initiation Site. \textit{Chemistry - A European Journal}. Weinheim: WILEY-VCH Verlag, 2009, roč.~15/2009, č.~46, s.~12663-12671, 8 s. ISSN~0947-6539.

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