J 2009

) Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

MRAVEC, Jozef; Petr SKŮPA; Aurélien BAILLY; Klára HOYEROVÁ; Pavel KŘEČEK et. al.

Základní údaje

Originální název

) Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

Název česky

) Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

Autoři

MRAVEC, Jozef; Petr SKŮPA; Aurélien BAILLY; Klára HOYEROVÁ; Pavel KŘEČEK; Agnieszka BIELACH; Jan PETRÁŠEK; Jing ZHANG; Vassilena GAYKOVA; York-Dieter STIERHOF; Petre I. DOBREV; Kateřina SCHWARZEROVÁ; Jakub ROLČÍK; Daniela SEIFERTOVÁ; Christian LUSCHNIG; Eva BENKOVÁ; Eva ZAŽÍMALOVÁ; Markus GEISLER a Jiří FRIML

Vydání

Nature, Lodon, UK, Nature Publishing Group, 2009, 0028-0836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 34.480

Kód RIV

RIV/00216224:14310/09:00028676

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000267636700045

Klíčová slova česky

auxin; PIN5; polar auxin transport; endoplasmic reticulum; subcellular homeostasis

Klíčová slova anglicky

auxin; PIN5; polar auxin transport; endoplasmic reticulum; subcellular homeostasis
Změněno: 3. 12. 2009 16:18, Mgr. Gabriela Jalová

Anotace

V originále

The plant signalling molecule auxin provides positional information in a variety of developmental processes by means of its differential distribution (gradients) within plant tissues. Thus, cellular auxin levels often determine the developmental output of auxin signalling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell. In particular, PIN auxin-efflux-carrier-mediated, directional transport between cells is crucial for generating auxin gradients. Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development. PIN5 does not have a direct role in cell-to-cell transport but regulates intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmic reticulum (ER), presumably mediating auxin flow from the cytosol to the lumen of the ER. The ER localization of other PIN5-like transporters (including the moss PIN) indicates that the diversification of PIN protein functions in mediating auxin homeostasis at the ER, and cell-to-cell auxin transport at the plasma membrane, represent an ancient event during the evolution of land plants.

Česky

The plant signalling molecule auxin provides positional information in a variety of developmental processes by means of its differential distribution (gradients) within plant tissues. Thus, cellular auxin levels often determine the developmental output of auxin signalling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell. In particular, PIN auxin-efflux-carrier-mediated, directional transport between cells is crucial for generating auxin gradients. Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development. PIN5 does not have a direct role in cell-to-cell transport but regulates intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmic reticulum (ER), presumably mediating auxin flow from the cytosol to the lumen of the ER. The ER localization of other PIN5-like transporters (including the moss PIN) indicates that the diversification of PIN protein functions in mediating auxin homeostasis at the ER, and cell-to-cell auxin transport at the plasma membrane, represent an ancient event during the evolution of land plants.

Návaznosti

IAA601630703, projekt VaV
Název: Identifikace molekulárních komponent a mechanizmu polárního směrování auxinových transportních proteinů rodiny PIN u Arabidopsis thaliana
Investor: Akademie věd ČR, Identifikace molekulárních komponent a mechanizmu polárního směrování auxinových transportních proteinů rodiny PIN u Arabidopsis thaliana