Screening of macrolide resistance locus in Treponema pallidum
subsp. pallidum in the Czech Republic.

D 2009

Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic.

MATĚJKOVÁ POSPÍŠILOVÁ, Petra; Magdalena FLASAROVÁ; Hana ZÁKOUCKÁ; M. BOŘEK; Gabriela KŘEMENOVÁ et al.

Základní údaje

Originální název

Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic.

Autoři

MATĚJKOVÁ POSPÍŠILOVÁ, Petra; Magdalena FLASAROVÁ; Hana ZÁKOUCKÁ; M. BOŘEK; Gabriela KŘEMENOVÁ; P. ARENBERGER; Vladana WOZNICOVÁ; G. M. WEINSTOCK a David ŠMAJS

Vydání

Neuveden, Clinical Microbiology and Infection, Abstracts of the 19th ECCMID, P698, s. 168-168, 2009

Nakladatel

European Society of Clinical Microbiology and Infectious Diseases

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Finsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.014

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISSN

Klíčová slova česky

makrolidová rezistence; 23S rRNA; syfilis

Klíčová slova anglicky

macrolide resistance; 23S rRNA; syphilis

Změněno: 24. 2. 2010 18:04, Mgr. Marie Zobaníková, Ph.D.

Anotace

V originále

Due to alergies to penicillin, macrolide regimens are frequently applied in the treatment of syphilis. A2058G mutation in the 23S rDNA locus was described to cause erythromycin resistance in syphilis.Molecular detection of syphilis represents the only available test to reveal macrolide resistance in clinical isolates, because T. pallidum can not be cultured in vitro. The set of 28 clinical isolates with detectable genetic material were collected from 22 patients in the Czech Republic in the time period 2005 to 2008. Four patients (18.2%) were diagnosed with a strain bearing the A2058G transition and four patients (18.2%) with a strain bearing the A2059G mutation. Screening of clinical samples revealed a novel mutation at the position 2059 of the T. pallidum 23S rRNA gene that causes macrolide resistance in several other bacteria. Our results show that macrolide resistant isolates of T. pallidum bearing A2058G and A2059G mutations are relatively abundant in the Czech Republic.

Návaznosti

GA310/07/0321, projekt VaV

Název: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Grantová agentura ČR, Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

NR8967, projekt VaV

Název: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministerstvo zdravotnictví ČR, Molekulární typizace kmenů a izolátů Treponema pallidum subsp. Pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Citovat

MATĚJKOVÁ POSPÍŠILOVÁ, Petra; Magdalena FLASAROVÁ; Hana ZÁKOUCKÁ; M. BOŘEK; Gabriela KŘEMENOVÁ; P. ARENBERGER; Vladana WOZNICOVÁ; G. M. WEINSTOCK a David ŠMAJS. Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic. In Clinical Microbiology and Infection, Abstracts of the 19th ECCMID, P698. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2009, s. 168. ISSN 1198-743X.

@inproceedings{873304,
   author = {Matějková Pospíšilová, Petra and Flasarová, Magdalena and Zákoucká, Hana and Bořek, M. and Křemenová, Gabriela and Arenberger, P. and Woznicová, Vladana and Weinstock, G. M. and Šmajs, David},
   address = {Neuveden},
   booktitle = {Clinical Microbiology and Infection, Abstracts of the 19th ECCMID, P698},
   keywords = {macrolide resistance; 23S rRNA; syphilis},
   language = {eng},
   location = {Neuveden},
   pages = {168-168},
   publisher = {European Society of Clinical Microbiology and Infectious Diseases},
   title = {Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic.},
   url = {http://www3.interscience.wiley.com/cgi-bin/fulltext/122401282/PDFSTART},
   year = {2009}
}

TY  - CONF
ID  - 873304
AU  - Matějková Pospíšilová, Petra - Flasarová, Magdalena - Zákoucká, Hana - Bořek, M. - Křemenová, Gabriela - Arenberger, P. - Woznicová, Vladana - Weinstock, G. M. - Šmajs, David
PY  - 2009
TI  - Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic.
PB  - European Society of Clinical Microbiology and Infectious Diseases
CY  - Neuveden
KW  - macrolide resistance
KW  - 23S rRNA
KW  - syphilis
UR  - http://www3.interscience.wiley.com/cgi-bin/fulltext/122401282/PDFSTART
N2  - Due to alergies to penicillin, macrolide regimens are frequently applied in the treatment of syphilis. A2058G mutation in the 23S rDNA locus was described to cause erythromycin resistance in syphilis.Molecular detection of syphilis represents the only available test to reveal macrolide resistance in clinical isolates, because T. pallidum can not be cultured in vitro. The set of 28 clinical isolates with detectable genetic material were collected from 22 patients in the Czech Republic in the time period 2005 to 2008. Four patients (18.2%) were diagnosed with a strain bearing the A2058G transition and four patients (18.2%) with a strain bearing the A2059G mutation. Screening of clinical samples revealed a novel mutation at the position 2059 of the T. pallidum 23S rRNA gene that causes macrolide resistance in several other bacteria. Our results show that macrolide resistant isolates of T. pallidum bearing A2058G and A2059G mutations are relatively abundant in the Czech Republic.
ER  -

MATĚJKOVÁ POSPÍŠILOVÁ, Petra; Magdalena FLASAROVÁ; Hana ZÁKOUCKÁ; M. BOŘEK; Gabriela KŘEMENOVÁ; P. ARENBERGER; Vladana WOZNICOVÁ; G. M. WEINSTOCK a David ŠMAJS. Screening of macrolide resistance locus in Treponema pallidum subsp. pallidum in the Czech Republic. In \textit{Clinical Microbiology and Infection, Abstracts of the 19th ECCMID, P698}. Neuveden: European Society of Clinical Microbiology and Infectious Diseases, 2009, s.~168. ISSN~1198-743X.

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