Signalling mechanisms involved in the inhibition of gap-junctional intercellular communication by cyanobacterial extracts

ADAMOVSKÝ, Ondřej, Luděk BLÁHA, Pavel BABICA, Klára HILSCHEROVÁ a Brad UPHAM. Signalling mechanisms involved in the inhibition of gap-junctional intercellular communication by cyanobacterial extracts. In The 8th international conference on toxic cyanobacteria, Istanbul, Turkey. 2010.

Základní údaje

• Originální název: Signalling mechanisms involved in the inhibition of gap-junctional intercellular communication by cyanobacterial extracts
• Název česky: Signalní mechanismy ovlivněné inhibicí mezibuňečné komunikace sinicovými toxiny
• Autoři: ADAMOVSKÝ, Ondřej (203 Česká republika, garant), Luděk BLÁHA (203 Česká republika), Pavel BABICA (203 Česká republika), Klára HILSCHEROVÁ (203 Česká republika) a Brad UPHAM (840 Spojené státy).
• Vydání: The 8th international conference on toxic cyanobacteria, Istanbul, Turkey, 2010.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 30304 Public and environmental health
• Stát vydavatele: Turecko
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/10:00048814
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: sinocové toxiny promoce karcinogeneze
• Klíčová slova anglicky: cyanobacterial toxins tumor promotion

Anotace

We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on the inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Modulation of both parameters is an important in vitro biomarker of tumor promotional potencies. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. We have also investigated possible mechanisms controlling the inhibition of GJIC by cyanobacterial extract by using inhibitors of different signaling pathways such as PC-PLC, ERK, PKA and others.

Anotace česky

We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on the inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Modulation of both parameters is an important in vitro biomarker of tumor promotional potencies. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. We have also investigated possible mechanisms controlling the inhibition of GJIC by cyanobacterial extract by using inhibitors of different signaling pathways such as PC-PLC, ERK, PKA and others.

Návaznosti

• GA524/08/0496, projekt VaV: Název: Mechanismy nádorové promoce metabolitů toxických sinic

Investor: Grantová agentura ČR, Mechanismy nádorové promoce metabolitů toxických sinic