MOTÁČKOVÁ, Veronika, Jiří NOVÁČEK, Anna ZAWADZKA-KAZIMIERCZUK, Krzysztof KAZIMIERCZUK, Lukáš ŽÍDEK, Hana ŠANDEROVÁ, Libor KRÁSNÝ, Wiktor KOZMINSKI a Vladimír SKLENÁŘ. Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments. Online. Journal of Biomolecular NMR. Netherlands: Springer Netherlands, 2010, roč. 48, č. 3, s. 169-177. ISSN 0925-2738. [citováno 2024-04-24] |
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@article{903212, author = {Motáčková, Veronika and Nováček, Jiří and ZawadzkaandKazimierczuk, Anna and Kazimierczuk, Krzysztof and Žídek, Lukáš and Šanderová, Hana and Krásný, Libor and Kozminski, Wiktor and Sklenář, Vladimír}, article_location = {Netherlands}, article_number = {3}, keywords = {Unfolded proteins; Repetitive sequence; Multi-dimensional NMR; Non-uniform sampling; Assignment}, language = {eng}, issn = {0925-2738}, journal = {Journal of Biomolecular NMR}, title = {Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments}, url = {http://www.springerlink.com/content/?k=doi%3a%2810.1007%2fs10858-010-9447-3%29}, volume = {48}, year = {2010} }
TY - JOUR ID - 903212 AU - Motáčková, Veronika - Nováček, Jiří - Zawadzka-Kazimierczuk, Anna - Kazimierczuk, Krzysztof - Žídek, Lukáš - Šanderová, Hana - Krásný, Libor - Kozminski, Wiktor - Sklenář, Vladimír PY - 2010 TI - Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments JF - Journal of Biomolecular NMR VL - 48 IS - 3 SP - 169-177 EP - 169-177 PB - Springer Netherlands SN - 09252738 KW - Unfolded proteins KW - Repetitive sequence KW - Multi-dimensional NMR KW - Non-uniform sampling KW - Assignment UR - http://www.springerlink.com/content/?k=doi%3a%2810.1007%2fs10858-010-9447-3%29 N2 - A strategy for complete backbone and side-chain resonance assignment of disordered proteins with highly repetitive sequence is presented. The protocol is based on three resolution-enhanced NMR experiments: 5D HN(CA)CONH provides sequential connectivity, 5D HabCabCONH is utilized to identify amino acid types, and 5D HC(CC-TOCSY)CONH is used to assign the side-chain resonances. The improved resolution was achieved by a combination of high dimensionality and long evolution times, allowed by non-uniform sampling in the indirect dimensions. Random distribution of the data points and Sparse Multidimensional Fourier Transform processing were used. Successful application of the assignment procedure to a particularly difficult protein, delta subunit of RNA polymerase from Bacillus subtilis, is shown to prove the efficiency of the strategy. The studied protein contains a disordered C-terminal region of 81 amino acids with a highly repetitive sequence. While the conventional assignment methods completely failed due to a very small differences in chemical shifts, the presented strategy provided a complete backbone and side-chain assignment. ER -
MOTÁČKOVÁ, Veronika, Jiří NOVÁČEK, Anna ZAWADZKA-KAZIMIERCZUK, Krzysztof KAZIMIERCZUK, Lukáš ŽÍDEK, Hana ŠANDEROVÁ, Libor KRÁSNÝ, Wiktor KOZMINSKI a Vladimír SKLENÁŘ. Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments. Online. \textit{Journal of Biomolecular NMR}. Netherlands: Springer Netherlands, 2010, roč.~48, č.~3, s.~169-177. ISSN~0925-2738. [citováno 2024-04-24]
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