Antibiotic resistance in faecal bacteria (Escherichia coli,
Enterococcus spp.) in feral pigeons

J 2010

Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

RADIMĚŘSKÝ, Tomáš, Petra FROLKOVÁ, Dagmar JANOSZOWSKÁ, Monika DOLEJSKÁ, Pavel ŠVEC et. al.

Základní údaje

Originální název

Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

Autoři

RADIMĚŘSKÝ, Tomáš (203 Česká republika), Petra FROLKOVÁ (203 Česká republika, garant), Dagmar JANOSZOWSKÁ (203 Česká republika), Monika DOLEJSKÁ (203 Česká republika), Pavel ŠVEC (203 Česká republika, domácí), Eva ROUBALOVÁ (203 Česká republika), Petra ČÍKOVÁ (203 Česká republika), Alois ČÍŽEK (203 Česká republika) a Ivan LITERÁK (203 Česká republika)

Vydání

Journal of Applied Microbiology, Oxford, UK, Blackwell Science Ltd, 2010, 1364-5072

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.365

Kód RIV

RIV/00216224:14310/10:00045191

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000282813800020

Klíčová slova anglicky

antimicrobials; Columba livia; Czech Republic; genes; intestinal bacteria; resistance

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 2. 2013 15:01, RNDr. Eva Roubalová, Ph.D.

Anotace

V originále

Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) in the Czech Republic. Methods and Results: Cloacal swabs of feral pigeons collected in the city of Brno in 2006 were cultivated for antibiotic-resistant E. coli. Resistance genes, class 1 and 2 integrons, and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). The samples were also cultivated for enterococci. Species status of enterococci isolates was determined using repetitive extragenic palindromic-PCR. Resistance genes were detected in resistant enterococci by PCR. E. coli isolates were found in 203 of 247 pigeon samples. Antibiotic resistance was recorded in three (1.5%, nE. coli = 203) isolates. Using agar containing ciprofloxacin, 12 (5%, nsamples = 247) E. coli strains resistant to ciprofloxacin were isolated. No ESBL-producing E. coli isolates were detected. A total of 143 enterococci were isolated: Ent. faecalis (36 isolates), Ent. faecium (27), Ent. durans (19), Ent. hirae (17), Ent. mundtii (17), Ent. gallinarum (12), Ent. casseliflavus (12) and Ent. columbae (3). Resistance to one to four antibiotics was detected in 45 (31%) isolates. Resistances were determined by tetK, tetL, tetM, tetO, aac(6')aph(2''), ant(4')-Ia, aph(3')-IIIa, ermB, pbp5, vanA and vanC1 genes. Conclusions: Antibiotic-resistant E. coli and Enterococcus spp. occurred in feral pigeons in various prevalences. Significance and Impact of the Study: Feral pigeon should be considered a risk species for spreading in the environment antimicrobial resistant E. coli and enterococci.

Návaznosti

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

RADIMĚŘSKÝ, Tomáš, Petra FROLKOVÁ, Dagmar JANOSZOWSKÁ, Monika DOLEJSKÁ, Pavel ŠVEC, Eva ROUBALOVÁ, Petra ČÍKOVÁ, Alois ČÍŽEK a Ivan LITERÁK. Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons. Journal of Applied Microbiology. Oxford, UK: Blackwell Science Ltd, 2010, roč. 109, č. 5, s. 1687-1695. ISSN 1364-5072.

@article{904528,
   author = {Radiměřský, Tomáš and Frolková, Petra and Janoszowská, Dagmar and Dolejská, Monika and Švec, Pavel and Roubalová, Eva and Číková, Petra and Čížek, Alois and Literák, Ivan},
   article_location = {Oxford, UK},
   article_number = {5},
   keywords = {antimicrobials; Columba livia; Czech Republic; genes; intestinal bacteria; resistance},
   language = {eng},
   issn = {1364-5072},
   journal = {Journal of Applied Microbiology},
   title = {Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04797.x/abstract},
   volume = {109},
   year = {2010}
}

TY  - JOUR
ID  - 904528
AU  - Radiměřský, Tomáš - Frolková, Petra - Janoszowská, Dagmar - Dolejská, Monika - Švec, Pavel - Roubalová, Eva - Číková, Petra - Čížek, Alois - Literák, Ivan
PY  - 2010
TI  - Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons
JF  - Journal of Applied Microbiology
VL  - 109
IS  - 5
SP  - 1687-1695
EP  - 1687-1695
PB  - Blackwell Science Ltd
SN  - 13645072
KW  - antimicrobials
KW  - Columba livia
KW  - Czech Republic
KW  - genes
KW  - intestinal bacteria
KW  - resistance
UR  - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04797.x/abstract
N2  - Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) in the Czech Republic. Methods and Results: Cloacal swabs of feral pigeons collected in the city of Brno in 2006 were cultivated for antibiotic-resistant E. coli. Resistance genes, class 1 and 2 integrons, and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). The samples were also cultivated for enterococci. Species status of enterococci isolates was determined using repetitive extragenic palindromic-PCR. Resistance genes were detected in resistant enterococci by PCR. E. coli isolates were found in 203 of 247 pigeon samples. Antibiotic resistance was recorded in three (1.5%, nE. coli = 203) isolates. Using agar containing ciprofloxacin, 12 (5%, nsamples = 247) E. coli strains resistant to ciprofloxacin were isolated. No ESBL-producing E. coli isolates were detected. A total of 143 enterococci were isolated: Ent. faecalis (36 isolates), Ent. faecium (27), Ent. durans (19), Ent. hirae (17), Ent. mundtii (17), Ent. gallinarum (12), Ent. casseliflavus (12) and Ent. columbae (3). Resistance to one to four antibiotics was detected in 45 (31%) isolates. Resistances were determined by tetK, tetL, tetM, tetO, aac(6')aph(2''), ant(4')-Ia, aph(3')-IIIa, ermB, pbp5, vanA and vanC1 genes. Conclusions: Antibiotic-resistant E. coli and Enterococcus spp. occurred in feral pigeons in various prevalences. Significance and Impact of the Study: Feral pigeon should be considered a risk species for spreading in the environment antimicrobial resistant E. coli and enterococci.
ER  -

RADIMĚŘSKÝ, Tomáš, Petra FROLKOVÁ, Dagmar JANOSZOWSKÁ, Monika DOLEJSKÁ, Pavel ŠVEC, Eva ROUBALOVÁ, Petra ČÍKOVÁ, Alois ČÍŽEK a Ivan LITERÁK. Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons. \textit{Journal of Applied Microbiology}. Oxford, UK: Blackwell Science Ltd, 2010, roč.~109, č.~5, s.~1687-1695. ISSN~1364-5072.

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