Development of humanized culture medium with plant-derived
serum replacement for human pluripotent stem cells

J 2010

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells

KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Petra TRČKOVÁ, Aleš HAMPL et. al.

Základní údaje

Originální název

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells

Autoři

KUNOVÁ, Michaela (203 Česká republika, garant, domácí), Kamil MATULKA (203 Česká republika, domácí), Lívia EISELLEOVÁ (703 Slovensko, domácí), Petra TRČKOVÁ (203 Česká republika, domácí), Aleš HAMPL (203 Česká republika) a Petr DVOŘÁK (203 Česká republika)

Vydání

Reproductive BioMedicine Online, 2010, 1472-6483

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.285

Kód RIV

RIV/00216224:14110/10:00046015

Organizační jednotka

Lékařská fakulta

UT WoS

000284797000016

Klíčová slova anglicky

animal protein-free culture; high-density culture; human embryonic stem cells; humanized culture medium; plant-derived serum supplement; VegetaCell

Změněno: 1. 8. 2013 11:33, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy.

Citovat

KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Petra TRČKOVÁ, Aleš HAMPL a Petr DVOŘÁK. Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells. Reproductive BioMedicine Online. 2010, roč. 21, č. 5, s. 76-86. ISSN 1472-6483.

@article{914620,
   author = {Kunová, Michaela and Matulka, Kamil and Eiselleová, Lívia and Trčková, Petra and Hampl, Aleš and Dvořák, Petr},
   article_number = {5},
   keywords = {animal protein-free culture; high-density culture; human embryonic stem cells; humanized culture medium; plant-derived serum supplement; VegetaCell},
   language = {eng},
   issn = {1472-6483},
   journal = {Reproductive BioMedicine Online},
   title = {Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells},
   volume = {21},
   year = {2010}
}

TY  - JOUR
ID  - 914620
AU  - Kunová, Michaela - Matulka, Kamil - Eiselleová, Lívia - Trčková, Petra - Hampl, Aleš - Dvořák, Petr
PY  - 2010
TI  - Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells
JF  - Reproductive BioMedicine Online
VL  - 21
IS  - 5
SP  - 76-86
EP  - 76-86
SN  - 14726483
KW  - animal protein-free culture
KW  - high-density culture
KW  - human embryonic stem cells
KW  - humanized culture medium
KW  - plant-derived serum supplement
KW  - VegetaCell
N2  - For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy.
ER  -

KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Petra TRČKOVÁ, Aleš HAMPL a Petr DVOŘÁK. Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells. \textit{Reproductive BioMedicine Online}. 2010, roč.~21, č.~5, s.~76-86. ISSN~1472-6483.

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