HRUDA, Jan, Vladimír ŠRÁMEK and Xavier LEVERVE. HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS. Biomedical Papers. Olomouc: Palacky University, 2010, vol. 154, No 4, p. 315-320. ISSN 1213-8118. |
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@article{920781, author = {Hruda, Jan and Šrámek, Vladimír and Leverve, Xavier}, article_location = {Olomouc}, article_number = {4}, keywords = {K-562 cells; Oxidative stress; Apoptosis; Energy substrates; Hyperglycemia; DNA damage}, language = {eng}, issn = {1213-8118}, journal = {Biomedical Papers}, title = {HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS}, volume = {154}, year = {2010} }
TY - JOUR ID - 920781 AU - Hruda, Jan - Šrámek, Vladimír - Leverve, Xavier PY - 2010 TI - HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS JF - Biomedical Papers VL - 154 IS - 4 SP - 315-320 EP - 315-320 PB - Palacky University SN - 12138118 KW - K-562 cells KW - Oxidative stress KW - Apoptosis KW - Energy substrates KW - Hyperglycemia KW - DNA damage N2 - The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. Methods. Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic “DNA comets” assay at 5 mM and 30 mM glucose concentrations. Results. The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant – N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. Conclusion. Hyperglycemia increases the cell’s susceptibility to oxidative stress and it also amplifies oxidative DNA damage. Glutamine – when used as a sole energetic substrate – showed no protective effect against oxidative stress. ER -
HRUDA, Jan, Vladimír ŠRÁMEK and Xavier LEVERVE. HIGH GLUCOSE INCREASES SUSCEPTIBILITY TO OXIDATIVE-STRESS-INDUCED APOPTOSIS AND DNA DAMAGE IN K-562 CELLS. \textit{Biomedical Papers}. Olomouc: Palacky University, 2010, vol.~154, No~4, p.~315-320. ISSN~1213-8118.
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