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@article{920883, author = {Vlková, Marcela and Froňková, E. and Kanderová, V. and Janda, A. and Růžičková, Š. and Litzman, Jiří and Šedivá, A. and Kalina, T.}, article_location = {Bethesda}, article_number = {11}, doi = {http://dx.doi.org/10.4049/jimmunol.0903876}, keywords = {B cells; flow cytometry; CVID}, language = {eng}, issn = {0022-1767}, journal = {Journal of immunology}, title = {Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression}, volume = {185}, year = {2010} }
TY - JOUR ID - 920883 AU - Vlková, Marcela - Froňková, E. - Kanderová, V. - Janda, A. - Růžičková, Š. - Litzman, Jiří - Šedivá, A. - Kalina, T. PY - 2010 TI - Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression JF - Journal of immunology VL - 185 IS - 11 SP - 6431-6438 EP - 6431-6438 PB - American association of immunologists SN - 00221767 KW - B cells KW - flow cytometry KW - CVID N2 - Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. Consistent with previous studies, we have described two groups of patients with normal (CVID 21norm) or increased (CVID 21lo) proportions of CD27negCD21negCD38neg B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. Furthermore, when we analyzed CD21pos naive B cells, we found two different subpopulations based on IgM and CD24 expression. They correspond to follicular (FO) I and FO II cells previously described in mice. FO I subset is significantly underrepresented in CVID 21lo patients. A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression. ER -
VLKOVÁ, Marcela, E. FROŇKOVÁ, V. KANDEROVÁ, A. JANDA, Š. RŮŽIČKOVÁ, Jiří LITZMAN, A. ŠEDIVÁ a T. KALINA. Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. \textit{Journal of immunology}. Bethesda: American association of immunologists, 2010, roč.~185, č.~11, s.~6431-6438. ISSN~0022-1767. Dostupné z: https://dx.doi.org/10.4049/jimmunol.0903876.
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