In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain

JING, Ying, Ondřej MACHOŇ, Aleš HAMPL, Petr DVOŘÁK, Ying XING a Stefan KRAUSS. In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain. Cellular and Molecular Neurobiology. Springer, 2011, roč. 31, č. 5, s. 715-727. ISSN 0272-4340. Dostupné z: https://dx.doi.org/10.1007/s10571-011-9669-2.

Základní údaje

• Originální název: In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain
• Autoři: JING, Ying (156 Čína), Ondřej MACHOŇ (203 Česká republika), Aleš HAMPL (203 Česká republika, domácí), Petr DVOŘÁK (203 Česká republika, domácí), Ying XING (156 Čína) a Stefan KRAUSS (578 Norsko, garant).
• Vydání: Cellular and Molecular Neurobiology, Springer, 2011, 0272-4340.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30000 3. Medical and Health Sciences
• Stát vydavatele: Spojené státy
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 1.969
• Kód RIV: RIV/00216224:14110/11:00052566
• Organizační jednotka: Lékařská fakulta
• Doi: http://dx.doi.org/10.1007/s10571-011-9669-2
• UT WoS: 000290914300008
• Klíčová slova anglicky: Mouse; embryonic stem cell; Embryonic forebrain; Stem cell; differentiation; Wnt signaling
• Příznaky: Mezinárodní význam

Anotace

Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 10–12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies. As shown by immunohistochemistry and quantitative qRT-PCR, D6-GFP-positive cells from qEBs expressed genes that are characteristic for the dorsal forebrain such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. qEBs culture allowed the formation of a D6-GFP positive pseudo-polarized neuroepithelium with the characteristic presence of N-cadherin at the apical pole resembling the structure of the developing neocortex.