In Vitro Differentiation of Mouse Embryonic Stem Cells into
Neurons of the Dorsal Forebrain

J 2011

In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain

JING, Ying, Ondřej MACHOŇ, Aleš HAMPL, Petr DVOŘÁK, Ying XING et. al.

Základní údaje

Originální název

In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain

Autoři

JING, Ying (156 Čína), Ondřej MACHOŇ (203 Česká republika), Aleš HAMPL (203 Česká republika, domácí), Petr DVOŘÁK (203 Česká republika, domácí), Ying XING (156 Čína) a Stefan KRAUSS (578 Norsko, garant)

Vydání

Cellular and Molecular Neurobiology, Springer, 2011, 0272-4340

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.969

Kód RIV

RIV/00216224:14110/11:00052566

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s10571-011-9669-2

UT WoS

000290914300008

Klíčová slova anglicky

Mouse; embryonic stem cell; Embryonic forebrain; Stem cell; differentiation; Wnt signaling

Příznaky

Mezinárodní význam

Změněno: 1. 8. 2013 11:03, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 10–12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies. As shown by immunohistochemistry and quantitative qRT-PCR, D6-GFP-positive cells from qEBs expressed genes that are characteristic for the dorsal forebrain such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. qEBs culture allowed the formation of a D6-GFP positive pseudo-polarized neuroepithelium with the characteristic presence of N-cadherin at the apical pole resembling the structure of the developing neocortex.

Citovat

JING, Ying, Ondřej MACHOŇ, Aleš HAMPL, Petr DVOŘÁK, Ying XING a Stefan KRAUSS. In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain. Cellular and Molecular Neurobiology. Springer, 2011, roč. 31, č. 5, s. 715-727. ISSN 0272-4340. Dostupné z: https://dx.doi.org/10.1007/s10571-011-9669-2.

@article{943487,
   author = {Jing, Ying and Machoň, Ondřej and Hampl, Aleš and Dvořák, Petr and Xing, Ying and Krauss, Stefan},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s10571-011-9669-2},
   keywords = {Mouse; embryonic stem cell; Embryonic forebrain; Stem cell; differentiation; Wnt signaling},
   language = {eng},
   issn = {0272-4340},
   journal = {Cellular and Molecular Neurobiology},
   title = {In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain},
   volume = {31},
   year = {2011}
}

TY  - JOUR
ID  - 943487
AU  - Jing, Ying - Machoň, Ondřej - Hampl, Aleš - Dvořák, Petr - Xing, Ying - Krauss, Stefan
PY  - 2011
TI  - In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain
JF  - Cellular and Molecular Neurobiology
VL  - 31
IS  - 5
SP  - 715-727
EP  - 715-727
PB  - Springer
SN  - 02724340
KW  - Mouse
KW  - embryonic stem cell
KW  - Embryonic forebrain
KW  - Stem cell
KW  - differentiation
KW  - Wnt signaling
N2  - Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 10–12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFPpositive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies. As shown by immunohistochemistry and quantitative qRT-PCR, D6-GFP-positive cells from qEBs expressed genes that are characteristic for the dorsal forebrain such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. qEBs culture allowed the formation of a D6-GFP positive pseudo-polarized neuroepithelium with the characteristic presence of N-cadherin at the apical pole resembling the structure of the developing neocortex.
ER  -

JING, Ying, Ondřej MACHOŇ, Aleš HAMPL, Petr DVOŘÁK, Ying XING a Stefan KRAUSS. In Vitro Differentiation of Mouse Embryonic Stem Cells into Neurons of the Dorsal Forebrain. \textit{Cellular and Molecular Neurobiology}. Springer, 2011, roč.~31, č.~5, s.~715-727. ISSN~0272-4340. Dostupné z: https://dx.doi.org/10.1007/s10571-011-9669-2.

Podrobný výpis o publikaci