Další formáty:
BibTeX
LaTeX
RIS
@article{944323, author = {Horák, Daniel and Matulka, Kamil and Hlídková, Helena and Lapčíková, Monika and Beneš, Milan J. and Jaroš, Josef and Hampl, Aleš and Dvořák, Petr}, article_number = {1}, doi = {http://dx.doi.org/10.1002/jbm.b.31832}, keywords = {poly(NN-diethylacrylamide); porosity; YIGSR; peptide; embryonic stem cells; hydrogel}, language = {eng}, issn = {1552-4973}, journal = {Journal of Biomedical Materials Reseach Part B: Applied Biomaterials}, title = {Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering}, volume = {98B}, year = {2011} }
TY - JOUR ID - 944323 AU - Horák, Daniel - Matulka, Kamil - Hlídková, Helena - Lapčíková, Monika - Beneš, Milan J. - Jaroš, Josef - Hampl, Aleš - Dvořák, Petr PY - 2011 TI - Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering JF - Journal of Biomedical Materials Reseach Part B: Applied Biomaterials VL - 98B IS - 1 SP - 54-67 EP - 54-67 SN - 15524973 KW - poly(NN-diethylacrylamide) KW - porosity KW - YIGSR KW - peptide KW - embryonic stem cells KW - hydrogel N2 - Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes. ER -
HORÁK, Daniel, Kamil MATULKA, Helena HLÍDKOVÁ, Monika LAPČÍKOVÁ, Milan J. BENEŠ, Josef JAROŠ, Aleš HAMPL a Petr DVOŘÁK. Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering. \textit{Journal of Biomedical Materials Reseach Part B: Applied Biomaterials}. 2011, roč.~98B, č.~1, s.~54-67. ISSN~1552-4973. Dostupné z: https://dx.doi.org/10.1002/jbm.b.31832.
|