Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel
scaffolds for tissue engineering

J 2011

Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering

HORÁK, Daniel; Kamil MATULKA; Helena HLÍDKOVÁ; Monika LAPČÍKOVÁ; Milan J. BENEŠ et al.

Základní údaje

Originální název

Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering

Autoři

HORÁK, Daniel; Kamil MATULKA; Helena HLÍDKOVÁ; Monika LAPČÍKOVÁ; Milan J. BENEŠ; Josef JAROŠ; Aleš HAMPL a Petr DVOŘÁK

Vydání

Journal of Biomedical Materials Reseach Part B: Applied Biomaterials, 2011, 1552-4973

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.147

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/11:00052611

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1002/jbm.b.31832

UT WoS

000291598900007

Klíčová slova anglicky

poly(NN-diethylacrylamide); porosity; YIGSR; peptide; embryonic stem cells; hydrogel

Příznaky

Mezinárodní význam

Změněno: 1. 8. 2013 11:08, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.

Návaznosti

LC06077, projekt VaV

Název: Centrum chemické genetiky

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum chemické genetiky

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

HORÁK, Daniel; Kamil MATULKA; Helena HLÍDKOVÁ; Monika LAPČÍKOVÁ; Milan J. BENEŠ; Josef JAROŠ; Aleš HAMPL a Petr DVOŘÁK. Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering. Journal of Biomedical Materials Reseach Part B: Applied Biomaterials. 2011, roč. 98B, č. 1, s. 54-67. ISSN 1552-4973. Dostupné z: https://doi.org/10.1002/jbm.b.31832.

@article{944323,
   author = {Horák, Daniel and Matulka, Kamil and Hlídková, Helena and Lapčíková, Monika and Beneš, Milan J. and Jaroš, Josef and Hampl, Aleš and Dvořák, Petr},
   article_number = {1},
   doi = {https://doi.org/10.1002/jbm.b.31832},
   keywords = {poly(NN-diethylacrylamide); porosity; YIGSR; peptide; embryonic stem cells; hydrogel},
   language = {eng},
   issn = {1552-4973},
   journal = {Journal of Biomedical Materials Reseach Part B: Applied Biomaterials},
   title = {Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering},
   volume = {98B},
   year = {2011}
}

TY  - JOUR
ID  - 944323
AU  - Horák, Daniel - Matulka, Kamil - Hlídková, Helena - Lapčíková, Monika - Beneš, Milan J. - Jaroš, Josef - Hampl, Aleš - Dvořák, Petr
PY  - 2011
TI  - Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering
JF  - Journal of Biomedical Materials Reseach Part B: Applied Biomaterials
VL  - 98B
IS  - 1
SP  - 54-67
EP  - 54-67
SN  - 15524973
KW  - poly(NN-diethylacrylamide)
KW  - porosity
KW  - YIGSR
KW  - peptide
KW  - embryonic stem cells
KW  - hydrogel
N2  - Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.
ER  -

HORÁK, Daniel; Kamil MATULKA; Helena HLÍDKOVÁ; Monika LAPČÍKOVÁ; Milan J. BENEŠ; Josef JAROŠ; Aleš HAMPL a Petr DVOŘÁK. Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering. \textit{Journal of Biomedical Materials Reseach Part B: Applied Biomaterials}. 2011, roč.~98B, č.~1, s.~54-67. ISSN~1552-4973. Dostupné z: https://doi.org/10.1002/jbm.b.31832.

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