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@proceedings{950778,
author = {Beneš, Petr and Alexova, Petra and Knopfová, Lucia and Šmarda, Jan},
booktitle = {19th euroconference on apoptosis, Stocholm, Sweden, 14-17.9, 2011},
keywords = {wedelolactone; topoisomerase IIa; breast cancer},
language = {eng},
title = {The effects of wedelolactone on cancer cells depend on its redox state},
year = {2011}
}
TY - CONF
ID - 950778
AU - Beneš, Petr - Alexova, Petra - Knopfová, Lucia - Šmarda, Jan
PY - 2011
TI - The effects of wedelolactone on cancer cells depend on its redox state
KW - wedelolactone
KW - topoisomerase IIa
KW - breast cancer
N2 - The aim of this study was to further characterize the mechanism how wedelolactone affects topoisomerase IIa and cancer cells. Using electrophoretic mobility shift assay we found that wedelolactone inhibited binding of topoisomerase IIa to supercoiled plasmid DNA. The inhibitory effect of wedelolactone on the topoisomerase IIa was reversed by excess of enzyme but not DNA suggesting that wedelolactone exerted its inhibitory effect by interaction with the topoisomerase II protein. The in vitro inhibitory effect of wedelolactone on the topoisomerase IIa activity was redox-dependent as it diminished in the presence of reducing agents, such as DTT, glutathione, N-acetylcysteine and L-ascorbic acid. Similarly, cytotoxicity of wedelolactone in breast cancer MDA-MB-231 cells was inhibited by N-acetylcysteine but enhanced by buthionine sulfoximine, an inhibitor of glutathione synthesis. Finally, we found that wedelolactone can be oxidized in the presence of copper ions to semiquinone/quinone radicals.
ER -
BENEŠ, Petr, Petra ALEXOVA, Lucia KNOPFOVÁ a Jan ŠMARDA. The effects of wedelolactone on cancer cells depend on its redox state. In \textit{19th euroconference on apoptosis, Stocholm, Sweden, 14-17.9, 2011}. 2011.
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