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@proceedings{950780,
author = {Beneš, Petr and Jančeková, Blanka and Knopfová, Lucia and Šmarda, Jan},
booktitle = {Montreal International Symposium on angiogenesis and metastasis, Montreal, Kanada},
keywords = {cathepsin D; cell migration; breast cancer},
language = {eng},
title = {Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity},
year = {2011}
}
TY - CONF
ID - 950780
AU - Beneš, Petr - Jančeková, Blanka - Knopfová, Lucia - Šmarda, Jan
PY - 2011
TI - Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity
KW - cathepsin D
KW - cell migration
KW - breast cancer
N2 - The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases.
ER -
BENEŠ, Petr, Blanka JANČEKOVÁ, Lucia KNOPFOVÁ a Jan ŠMARDA. Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity. In \textit{Montreal International Symposium on angiogenesis and metastasis, Montreal, Kanada}. 2011.
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