The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver

DOSTÁLEK, Miroslav, Jana PISTOVČÁKOVÁ, Jan JUŘICA, Alexandra ŠULCOVÁ and Josef TOMANDL. The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver. Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic. 2011, vol. 155, No 3, p. 253-257. ISSN 1213-8118. Available from: https://dx.doi.org/10.5507/bp.2011.047.

Basic information

• Original name: The Effect of St. Johns Wort (Hypericum Perforatum) on Cytochrome P450 1A2 Activity in Perfused Rat Liver
• Authors: DOSTÁLEK, Miroslav (203 Czech Republic, belonging to the institution), Jana PISTOVČÁKOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution) and Josef TOMANDL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic, 2011, 1213-8118.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30104 Pharmacology and pharmacy
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: Biomed Pap 2011;155(3):253–258
• Impact factor: Impact factor: 0.702
• RIV identification code: RIV/00216224:14740/11:00050036
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.5507/bp.2011.047
• UT WoS: 000297807500005
• Keywords in English: St John‘s wort; Hypericum perforatum; Cytochrome P450; Liver isolated perfused; Rat
• Tags: ok, rivok
• Tags: International impact

Abstract

We previously reported the effect of St John’s wort (Hypericum perforatum) standardised extract LI 160 on the activities of cytochrome P450 2C6, 2D2 and 3As (Dostalek et al., Life Sciences 2005;78(3):239-44). In this study, we aimed to assess the effect of St John’s wort on the activity of cytochrome P450 1A2. The isolated perfused rat liver model was used in our experiments with phenacetin as a marker substrate for cytochrome P450 1A2. The rate of formation of acetaminophen was significantly inhibited by the use of St John’s wort. In addition, St John‘s wort extract inhibited cytochrome P450 1A2 activity significantly more than the control inhibitor. St John’s wort significantly inhibited cytochrome P450 1A2 enzyme activity. It remains to be determined whether the co-administration of St John‘s wort extract and other medications or substrates for cytochrome P450 1A2 could result in significant drug interactions.

Links

• GAP206/10/0057, research and development project: Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze

Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis

• LC06023, research and development project: Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.