| DOBEŠ, Pavel, Zhi WANG, Robert MARKUS, Ulrich THEOPOLD a Pavel HYRŠL. An improved method for nematode infection assays in Drosophila larvae. Online. Fly. Austin (USA): Landes Bioscience, 2012, roč. 6, č. 2, s. 75-79. ISSN 1933-6934. Dostupné z: https://dx.doi.org/10.4161/fly.19553. [citováno 2024-04-23] |
Další formáty:
BibTeX
LaTeX
RIS
@article{969196,
author = {Dobeš, Pavel and Wang, Zhi and Markus, Robert and Theopold, Ulrich and Hyršl, Pavel},
article_location = {Austin (USA)},
article_number = {2},
doi = {http://dx.doi.org/10.4161/fly.19553},
keywords = {Drosophila melanogaster; Galleria mellonella; entomopathogenic nematodes; Steinernema feltiae; Heterorhabditis bacteriophora},
language = {eng},
issn = {1933-6934},
journal = {Fly},
title = {An improved method for nematode infection assays in Drosophila larvae},
volume = {6},
year = {2012}
}
TY - JOUR
ID - 969196
AU - Dobeš, Pavel - Wang, Zhi - Markus, Robert - Theopold, Ulrich - Hyršl, Pavel
PY - 2012
TI - An improved method for nematode infection assays in Drosophila larvae
JF - Fly
VL - 6
IS - 2
SP - 75-79
EP - 75-79
PB - Landes Bioscience
SN - 19336934
KW - Drosophila melanogaster
KW - Galleria mellonella
KW - entomopathogenic nematodes
KW - Steinernema feltiae
KW - Heterorhabditis bacteriophora
N2 - The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity towards Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized. Using optimum conditions, a faster and efficient setup for nematode infections was developed. This newly established infection model in Drosophila larvae will be applicable in large scale screens aimed at identifying novel genes/pathways involved in innate immune responses.
ER -
DOBEŠ, Pavel, Zhi WANG, Robert MARKUS, Ulrich THEOPOLD a Pavel HYRŠL. An improved method for nematode infection assays in Drosophila larvae. Online. \textit{Fly}. Austin (USA): Landes Bioscience, 2012, roč.~6, č.~2, s.~75-79. ISSN~1933-6934. Dostupné z: https://dx.doi.org/10.4161/fly.19553. [citováno 2024-04-23]
|
