Early cycling-independent changes to p27, cyclin D2, and cyclin
D3 in differentiating mouse embryonal carcinoma cells.

J 2002

Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells.

PRECLÍKOVÁ, H.; Vítězslav BRYJA; Jiří PACHERNÍK; Pavel KREJČÍ; Petr DVOŘÁK et al.

Základní údaje

Originální název

Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells.

Název česky

Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells.

Autoři

PRECLÍKOVÁ, H.; Vítězslav BRYJA; Jiří PACHERNÍK; Pavel KREJČÍ; Petr DVOŘÁK a Aleš HAMPL

Vydání

Cell Growth and Differentiation, 2002, 1044-9523

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.642

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000178379000003

Klíčová slova anglicky

MESSENGER-RNA; STEM-CELLS; CDK INHIBITOR; GROWTH ARREST; TUMOR-CELLS; P19 CELLS; P27(KIP1)

Změněno: 15. 2. 2012 16:39, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

Changes to cell cycle-regulating machinery that occur during differentiation of cells are thought to be responsible mostly for withdrawal from cycling. Here, embryonal carcinoma (EC) cell lines were found that differ in their basal levels of p27 inhibitor of cyclin-dependent kinases but not in their growth rates' distribution of cells in phases of cell cycle, and their ability to differentiate. High basal levels of p27 did not substitute for upregulation of p27 that in EC cells normally occurs early after entering a differentiation pathway. Under both standard and differentiation-supporting culture conditions, variances in the levels of p27 were strictly followed by variances in the levels of cyclins D2 and D3. In EC cells genetically manipulated to overexpress p27 protein, cyclin D3 became up-regulated and vice versa. Supposedly, titration of p27 by D-type cyclins, which prevents its inhibitory action toward cyclin-dependent kinase 2, allows for the maintenance of elevated p27 in proliferating EC cells. Increased levels of p27 in early embryonal cells thus may, at least in certain phases of embryo development, serve a differentiation-associated, rather than proliferation-associated, function.

Citovat

PRECLÍKOVÁ, H.; Vítězslav BRYJA; Jiří PACHERNÍK; Pavel KREJČÍ; Petr DVOŘÁK a Aleš HAMPL. Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells. Cell Growth and Differentiation. 2002, roč. 130A, č. 9, s. 421-430. ISSN 1044-9523.

@article{971687,
   author = {Preclíková, H. and Bryja, Vítězslav and Pacherník, Jiří and Krejčí, Pavel and Dvořák, Petr and Hampl, Aleš},
   article_number = {9},
   keywords = {MESSENGER-RNA; STEM-CELLS; CDK INHIBITOR; GROWTH ARREST; TUMOR-CELLS; P19 CELLS; P27(KIP1)},
   language = {eng},
   issn = {1044-9523},
   journal = {Cell Growth and Differentiation},
   title = {Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells.},
   volume = {130A},
   year = {2002}
}

TY  - JOUR
ID  - 971687
AU  - Preclíková, H. - Bryja, Vítězslav - Pacherník, Jiří - Krejčí, Pavel - Dvořák, Petr - Hampl, Aleš
PY  - 2002
TI  - Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells.
JF  - Cell Growth and Differentiation
VL  - 130A
IS  - 9
SP  - 421-430
EP  - 421-430
SN  - 10449523
KW  - MESSENGER-RNA
KW  - STEM-CELLS
KW  - CDK INHIBITOR
KW  - GROWTH ARREST
KW  - TUMOR-CELLS
KW  - P19 CELLS
KW  - P27(KIP1)
N2  - Changes to cell cycle-regulating machinery that occur during differentiation of cells are thought to be responsible mostly for withdrawal from cycling. Here, embryonal carcinoma (EC) cell lines were found that differ in their basal levels of p27 inhibitor of cyclin-dependent kinases but not in their growth rates' distribution of cells in phases of cell cycle, and their ability to differentiate. High basal levels of p27 did not substitute for upregulation of p27 that in EC cells normally occurs early after entering a differentiation pathway. Under both standard and differentiation-supporting culture conditions, variances in the levels of p27 were strictly followed by variances in the levels of cyclins D2 and D3. In EC cells genetically manipulated to overexpress p27 protein, cyclin D3 became up-regulated and vice versa. Supposedly, titration of p27 by D-type cyclins, which prevents its inhibitory action toward cyclin-dependent kinase 2, allows for the maintenance of elevated p27 in proliferating EC cells. Increased levels of p27 in early embryonal cells thus may, at least in certain phases of embryo development, serve a differentiation-associated, rather than proliferation-associated, function.
ER  -

PRECLÍKOVÁ, H.; Vítězslav BRYJA; Jiří PACHERNÍK; Pavel KREJČÍ; Petr DVOŘÁK a Aleš HAMPL. Early cycling-independent changes to p27, cyclin D2, and cyclin D3 in differentiating mouse embryonal carcinoma cells. \textit{Cell Growth and Differentiation}. 2002, roč.~130A, č.~9, s.~421-430. ISSN~1044-9523.

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