O-linked carbohydrates are required for FGF-2-mediated
proliferation of mouse embryonic cells.

J 1999

O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells.

JIRMANOVA, L.; Jiřina PACHOLÍKOVÁ; Pavel KREJČÍ; A HAMPL; Petr DVOŘÁK et al.

Základní údaje

Originální název

O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells.

Autoři

JIRMANOVA, L.; Jiřina PACHOLÍKOVÁ; Pavel KREJČÍ; A HAMPL a Petr DVOŘÁK

Vydání

The International journal of developmental biology, Spain, University Of The Basque Country Press, 1999, 0214-6282

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.743

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000083773700009

Klíčová slova anglicky

STEM-CELLS; SULFATE PROTEOGLYCAN; ANTIGEN EXPRESSION; CARCINOMA-CELLS; SIALYL LEWIS(X); FGF BINDING; RECEPTOR; HEPARIN;

Změněno: 15. 2. 2012 17:02, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

During development, fibroblast growth factors (FGFs) serve highly specific functions that are mediated through high-affinity transmembrane receptors and modulated by membrane-bound proteoglycans. Proteoglycans, in an embryonic environment called embryoglycans, contain numerous carbohydrate ectodomains, the structure of which undergoes rearrangement. Since they can be lost from the cell surface, they are sometimes found in extracellular space where they may also serve some regulatory function. Here we address the potential roles of three naturally occurring isoforms of Lewis X (Le(X)) in FGF-2-mediated proliferation of embryonic stem (ES) cells. We have found that the addition of sulfated Le(X) to ES cells at a concentration of 17 nM promotes FGF-2 mitogenic activity while a 10-fold higher concentration leads to a reduction of FGF-2-mediated proliferation. Notably, this dose-dependent modulation operated only for sulfated Le(X). Other fucosylated motifs, basic Le(X) trisaccharide and sialylated Le(X), also affected ES cell proliferation but the mechanism cannot be clearly correlated with the presence or absence of FGF-2. The suppression of biosynthesis of O-linked carbohydrates including Le(X) reduced basal proliferation of ES cells and interfered with the mitogenic effect of FGF-2. However, in inhibitor-treated cells, the stimulatory activity of FGF-2 can be reestablished to its original level by exogenous Le(X) oligosaccharides. Our results show that (A) O-linked Le(X) oligosaccharides can regulate mitogenic activity of FGF-2 in embryonic cells, (B) and this ability varies with subtle modifications in their structure. Importantly, our data represent the first insight into the mechanism of how growth factor activities might be modulated by shedded embryoglycan ectodomains.

Citovat

JIRMANOVA, L.; Jiřina PACHOLÍKOVÁ; Pavel KREJČÍ; A HAMPL a Petr DVOŘÁK. O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells. The International journal of developmental biology. Spain: University Of The Basque Country Press, 1999, roč. 43, č. 6, s. 555-562. ISSN 0214-6282.

@article{971694,
   author = {Jirmanova, L. and Pacholíková, Jiřina and Krejčí, Pavel and Hampl, A and Dvořák, Petr},
   article_location = {Spain},
   article_number = {6},
   keywords = {STEM-CELLS; SULFATE PROTEOGLYCAN; ANTIGEN EXPRESSION; CARCINOMA-CELLS; SIALYL LEWIS(X); FGF BINDING; RECEPTOR; HEPARIN;},
   language = {eng},
   issn = {0214-6282},
   journal = {The International journal of developmental biology},
   title = {O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells.},
   volume = {43},
   year = {1999}
}

TY  - JOUR
ID  - 971694
AU  - Jirmanova, L. - Pacholíková, Jiřina - Krejčí, Pavel - Hampl, A - Dvořák, Petr
PY  - 1999
TI  - O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells.
JF  - The International journal of developmental biology
VL  - 43
IS  - 6
SP  - 555-562
EP  - 555-562
PB  - University Of The Basque Country Press
SN  - 02146282
KW  - STEM-CELLS
KW  - SULFATE PROTEOGLYCAN
KW  - ANTIGEN EXPRESSION
KW  - CARCINOMA-CELLS
KW  - SIALYL LEWIS(X)
KW  - FGF BINDING
KW  - RECEPTOR
KW  - HEPARIN;
N2  - During development, fibroblast growth factors (FGFs) serve highly specific functions that are mediated through high-affinity transmembrane receptors and modulated by membrane-bound proteoglycans. Proteoglycans, in an embryonic environment called embryoglycans, contain numerous carbohydrate ectodomains, the structure of which undergoes rearrangement. Since they can be lost from the cell surface, they are sometimes found in extracellular space where they may also serve some regulatory function. Here we address the potential roles of three naturally occurring isoforms of Lewis X (Le(X)) in FGF-2-mediated proliferation of embryonic stem (ES) cells. We have found that the addition of sulfated Le(X) to ES cells at a concentration of 17 nM promotes FGF-2 mitogenic activity while a 10-fold higher concentration leads to a reduction of FGF-2-mediated proliferation. Notably, this dose-dependent modulation operated only for sulfated Le(X). Other fucosylated motifs, basic Le(X) trisaccharide and sialylated Le(X), also affected ES cell proliferation but the mechanism cannot be clearly correlated with the presence or absence of FGF-2. The suppression of biosynthesis of O-linked carbohydrates including Le(X) reduced basal proliferation of ES cells and interfered with the mitogenic effect of FGF-2. However, in inhibitor-treated cells, the stimulatory activity of FGF-2 can be reestablished to its original level by exogenous Le(X) oligosaccharides. Our results show that (A) O-linked Le(X) oligosaccharides can regulate mitogenic activity of FGF-2 in embryonic cells, (B) and this ability varies with subtle modifications in their structure. Importantly, our data represent the first insight into the mechanism of how growth factor activities might be modulated by shedded embryoglycan ectodomains.
ER  -

JIRMANOVA, L.; Jiřina PACHOLÍKOVÁ; Pavel KREJČÍ; A HAMPL a Petr DVOŘÁK. O-linked carbohydrates are required for FGF-2-mediated proliferation of mouse embryonic cells. \textit{The International journal of developmental biology}. Spain: University Of The Basque Country Press, 1999, roč.~43, č.~6, s.~555-562. ISSN~0214-6282.

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