Detection and measurement of fungal burden in a guinea pig
model of invasive pulmonary aspergillosis by novel quantitative
nested real-time PCR compared with galactomannan and
(1,3)-beta-D-glucan detection.

J 2012

Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.

LENGEROVÁ, Martina; Iva KOCMANOVÁ; Zdeněk RÁČIL; Kristýna HRNČÍŘOVÁ; Šárka POSPÍŠILOVÁ et. al.

Základní údaje

Originální název

Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.

Autoři

LENGEROVÁ, Martina (203 Česká republika, garant, domácí); Iva KOCMANOVÁ (203 Česká republika, domácí); Zdeněk RÁČIL (203 Česká republika, domácí); Kristýna HRNČÍŘOVÁ (203 Česká republika, domácí); Šárka POSPÍŠILOVÁ (203 Česká republika, domácí); Jiří MAYER (203 Česká republika, domácí); Laura K NAJVAR (840 Spojené státy); Nathan P WIEDERHOLD (840 Spojené státy); William R KIRKPATRICK (840 Spojené státy) a Thomas F PATTERSON (840 Spojené státy)

Vydání

Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2012, 0095-1137

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.068

Kód RIV

RIV/00216224:14740/12:00058696

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1128/JCM.05356-11

UT WoS

000300997800011

Klíčová slova anglicky

Invasive pulmonary aspergillosis; real-time PCR; galactomannan; glucan

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 4. 2013 08:27, Olga Křížová

Anotace

V originále

We developed and assessed the diagnostic value of a novel quantitative nested real-time (QNRT) PCR assay targeting the internal transcribed spacer region of ribosomal DNA (rDNA) in a guinea pig model of invasive pulmonary aspergillosis. Groups of 5 immunosuppressed animals that were infected using an aerosol chamber with Aspergillus fumigatus conidia were humanely terminated 1 h postinoculation and at days 3,5,7,and 11 postchallenge, and lung tissue, bronchoalveolar lavage (BAL) fluid, whole blood, and serum samples were collected. The QNRT PCR results obtained with the serum and BAL fluid were compared to those achieved with galactomannan and (1,3)-beta-D-glucan assays. High fungal burden levels were detected by QNRT PCR in both lung tissue and BAL fluid in all infected animals at each time point, and the sensitivity of each assay in BAL fluid was 100% by day 3 and remained so through the remainder of the study. The sensitivity of detection of fungi in whole blood and serum samples was significantly lower, and some samples remained negative by all three assays despite the advanced stage of the infection. From these data, we can conclude that this novel QNRT PCR method was highly sensitive for the detection of A. fumigatus from different types of samples in this model. In addition, BAL fluid samples appeared to be the most suitable for the early diagnosis of invasive pulmonary aspergillosis. When testing serum, the use of a combination of available assays may increase the possibility of early detection of this opportunistic mycosis.

Návaznosti

FR-TI2/254, projekt VaV

Název: *Real-time PCR soupravy pro diagnostiku v onkologii (Akronym: ONKOKITY)

Investor: Ministerstvo průmyslu a obchodu ČR, Real-time PCR soupravy pro diagnostiku v onkologii

Citovat

LENGEROVÁ, Martina; Iva KOCMANOVÁ; Zdeněk RÁČIL; Kristýna HRNČÍŘOVÁ; Šárka POSPÍŠILOVÁ; Jiří MAYER; Laura K NAJVAR; Nathan P WIEDERHOLD; William R KIRKPATRICK a Thomas F PATTERSON. Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection. Journal of Clinical Microbiology. Washington: American Society for Microbiology, 2012, roč. 50, č. 3, s. 602-608. ISSN 0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.05356-11.

@article{980422,
   author = {Lengerová, Martina and Kocmanová, Iva and Ráčil, Zdeněk and Hrnčířová, Kristýna and Pospíšilová, Šárka and Mayer, Jiří and Najvar, Laura K and Wiederhold, Nathan P and Kirkpatrick, William R and Patterson, Thomas F},
   article_location = {Washington},
   article_number = {3},
   doi = {http://dx.doi.org/10.1128/JCM.05356-11},
   keywords = {Invasive pulmonary aspergillosis; real-time PCR; galactomannan; glucan},
   language = {eng},
   issn = {0095-1137},
   journal = {Journal of Clinical Microbiology},
   title = {Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/22189110},
   volume = {50},
   year = {2012}
}

TY  - JOUR
ID  - 980422
AU  - Lengerová, Martina - Kocmanová, Iva - Ráčil, Zdeněk - Hrnčířová, Kristýna - Pospíšilová, Šárka - Mayer, Jiří - Najvar, Laura K - Wiederhold, Nathan P - Kirkpatrick, William R - Patterson, Thomas F
PY  - 2012
TI  - Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.
JF  - Journal of Clinical Microbiology
VL  - 50
IS  - 3
SP  - 602-608
EP  - 602-608
PB  - American Society for Microbiology
SN  - 00951137
KW  - Invasive pulmonary aspergillosis
KW  - real-time PCR
KW  - galactomannan
KW  - glucan
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22189110
L2  - http://www.ncbi.nlm.nih.gov/pubmed/22189110
N2  - We developed and assessed the diagnostic value of a novel quantitative nested real-time (QNRT) PCR assay targeting the internal transcribed spacer region of ribosomal DNA (rDNA) in a guinea pig model of invasive pulmonary aspergillosis. Groups of 5 immunosuppressed animals that were infected using an aerosol chamber with Aspergillus fumigatus conidia were humanely terminated 1 h postinoculation and at days 3,5,7,and 11 postchallenge, and lung tissue, bronchoalveolar lavage (BAL) fluid, whole blood, and serum samples were collected. The QNRT PCR results obtained with the serum and BAL fluid were compared to those achieved with galactomannan and (1,3)-beta-D-glucan assays. High fungal burden levels were detected by QNRT PCR in both lung tissue and BAL fluid in all infected animals at each time point, and the sensitivity of each assay in BAL fluid was 100% by day 3 and remained so through the remainder of the study. The sensitivity of detection of fungi in whole blood and serum samples was significantly lower, and some samples remained negative by all three assays despite the advanced stage of the infection. From these data, we can conclude that this novel QNRT PCR method was highly sensitive for the detection of A. fumigatus from different types of samples in this model. In addition, BAL fluid samples appeared to be the most suitable for the early diagnosis of invasive pulmonary aspergillosis. When testing serum, the use of a combination of available assays may increase the possibility of early detection of this opportunistic mycosis.
ER  -

LENGEROVÁ, Martina; Iva KOCMANOVÁ; Zdeněk RÁČIL; Kristýna HRNČÍŘOVÁ; Šárka POSPÍŠILOVÁ; Jiří MAYER; Laura K NAJVAR; Nathan P WIEDERHOLD; William R KIRKPATRICK a Thomas F PATTERSON. Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection. \textit{Journal of Clinical Microbiology}. Washington: American Society for Microbiology, 2012, roč.~50, č.~3, s.~602-608. ISSN~0095-1137. Dostupné z: https://dx.doi.org/10.1128/JCM.05356-11.

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