MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the
DNA damage response in human embryonic stem cells (hESCs)

a 2012

MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the DNA damage response in human embryonic stem cells (hESCs)

DOLEŽALOVÁ, Dáša; Marek MRÁZ; Vladimír VINARSKÝ; Zuzana HOLUBCOVÁ; Josef JAROŠ et. al.

Základní údaje

Originální název

MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the DNA damage response in human embryonic stem cells (hESCs)

Autoři

DOLEŽALOVÁ, Dáša; Marek MRÁZ; Vladimír VINARSKÝ; Zuzana HOLUBCOVÁ; Josef JAROŠ; Petr DVOŘÁK; Šárka POSPÍŠILOVÁ a Aleš HAMPL

Vydání

ISSCR 10th Annual Meeting, 13.-.16.6.2012, Yokohama, Japonsko, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Stát vydavatele

Japonsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Příznaky

Mezinárodní význam

Změněno: 13. 8. 2012 11:38, Mgr. Martina Vráblíková

Anotace

V originále

Studies of human embryonic stem cells (hESCs) commonly describe the nonfunctional p53-p21 axis of the G1/S checkpoint pathway with subsequent relevance for cell cycle regulation and the DNA damage response (DDR). Importantly, p21 mRNA is clearly present and upregulated after the DDR in hESCs, but p21 protein is not detectable. In this article, we provide evidence that expression of p21 protein is directly regulated by the microRNA (miRNA) pathway under standard culture conditions and after DNA damage. The DDR in hESCs leads to upregulation of tens of miRNAs, including hESC-specific miRNAs such as those of the miR-302 family, miR-371-372 family, or C19MC miRNA cluster. Most importantly, we show that the hESC-enriched miRNA family miR-302 (miR-302a, miR-302b, miR-302c, and miR-302d) directly contributes to regulation of p21 expression in hESCs and, thus, demonstrate a novel function for miR-302s in hESCS. The described mechanism elucidates the role of miRNAs in regulation of important molecular pathway governing the G1/S transition checkpoint before as well as after DNA damage.

Citovat

DOLEŽALOVÁ, Dáša; Marek MRÁZ; Vladimír VINARSKÝ; Zuzana HOLUBCOVÁ; Josef JAROŠ; Petr DVOŘÁK; Šárka POSPÍŠILOVÁ a Aleš HAMPL. MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the DNA damage response in human embryonic stem cells (hESCs). In ISSCR 10th Annual Meeting, 13.-.16.6.2012, Yokohama, Japonsko. 2012.

@proceedings{989134,
   author = {Doležalová, Dáša and Mráz, Marek and Vinarský, Vladimír and Holubcová, Zuzana and Jaroš, Josef and Dvořák, Petr and Pospíšilová, Šárka and Hampl, Aleš},
   booktitle = {ISSCR 10th Annual Meeting, 13.-.16.6.2012, Yokohama, Japonsko},
   language = {eng},
   title = {MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the DNA damage response in human embryonic stem cells (hESCs)},
   year = {2012}
}

TY  - CONF
ID  - 989134
AU  - Doležalová, Dáša - Mráz, Marek - Vinarský, Vladimír - Holubcová, Zuzana - Jaroš, Josef - Dvořák, Petr - Pospíšilová, Šárka - Hampl, Aleš
PY  - 2012
TI  - MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the DNA damage response in human embryonic stem cells (hESCs)
N2  - Studies of human embryonic stem cells (hESCs) commonly describe the nonfunctional p53-p21 axis of the G1/S checkpoint pathway with subsequent relevance for cell cycle regulation and the DNA damage response (DDR). Importantly, p21 mRNA is clearly present and upregulated after the DDR in hESCs, but p21 protein is not detectable. In this article, we provide evidence that expression of p21 protein is directly regulated by the microRNA (miRNA) pathway under standard culture conditions and after DNA damage. The DDR in hESCs leads to upregulation of tens of miRNAs, including hESC-specific miRNAs such as those of the miR-302 family, miR-371-372 family, or C19MC miRNA cluster. Most importantly, we show that the hESC-enriched miRNA family miR-302 (miR-302a, miR-302b, miR-302c, and miR-302d) directly contributes to regulation of p21 expression in hESCs and, thus, demonstrate a novel function for miR-302s in hESCS. The described mechanism elucidates the role of miRNAs in regulation of important molecular pathway governing the G1/S transition checkpoint before as well as after DNA damage.
ER  -

DOLEŽALOVÁ, Dáša; Marek MRÁZ; Vladimír VINARSKÝ; Zuzana HOLUBCOVÁ; Josef JAROŠ; Petr DVOŘÁK; Šárka POSPÍŠILOVÁ a Aleš HAMPL. MicroRNAs regulate P21WAF1 / CIP 1 protein expression and the DNA damage response in human embryonic stem cells (hESCs). In \textit{ISSCR 10th Annual Meeting, 13.-.16.6.2012, Yokohama, Japonsko}. 2012.

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