2012
Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process
PEŠL, Martin; Ivana AĆIMOVIĆ; Stanislava KOŠKOVÁ; Jan VRBSKÝ; Petr DVOŘÁK et al.Základní údaje
Originální název
Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process
Název česky
DIFERENCIACE LIDSKÝCH EMBRYONÁLNÍCH KMENOVÝCH BUNĚK A LIDSKÝCH PLURIPOTENTNÍCH KMENOVÝCH BUNĚK V KARDIOMYOCYTY VE VYSOCE EFEKTIVNÍM PROCESU
Název anglicky
Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process
Autoři
Vydání
2012 Mayo Young Investigators Research Symposium, 2012
Další údaje
Typ výsledku
Konferenční abstrakt
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ne
Příznaky
Mezinárodní význam
Změněno: 21. 5. 2018 12:44, MUDr. Martin Pešl, Ph.D.
V originále
Rationale: Human induced pluripotent and embryonic stem cells (hiPS and hES) can be differentiated into cardiomyocytes (CMs) in vitro. Developed methods like cocultivation with END2 cells, differentiation on matrigel and also cytokine effect on clusters of cells, so called EB method. The current studies are facing very low yield and process variation according to the type of used stem cell line. Objective: The aim of this work is to generate human CMs from AggreWell plates through EB method. Methods and results: Cardiomyocytes were generated through modified protocol inspired by Gordon Keller s lab work(Toronto, Can). Subsequent analyzes of gene expression, imunohistochemistry and electrophysiology proved cardiomyocyte – like character of differentiated cells, neverthless the cells represent very young stage of developing heart and has to be further investigated. This basic research opens way for further pacient specific investigation on the level of cellular cardiology.
Anglicky
Rationale: Human induced pluripotent and embryonic stem cells (hiPS and hES) can be differentiated into cardiomyocytes (CMs) in vitro. Developed methods like cocultivation with END2 cells, differentiation on matrigel and also cytokine effect on clusters of cells, so called EB method. The current studies are facing very low yield and process variation according to the type of used stem cell line. Objective: The aim of this work is to generate human CMs from AggreWell plates through EB method. Methods and results: Cardiomyocytes were generated through modified protocol inspired by Gordon Keller s lab work(Toronto, Can). Subsequent analyzes of gene expression, imunohistochemistry and electrophysiology proved cardiomyocyte – like character of differentiated cells, neverthless the cells represent very young stage of developing heart and has to be further investigated. This basic research opens way for further pacient specific investigation on the level of cellular cardiology.
Návaznosti
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