Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process

PEŠL, Martin, Ivana AĆIMOVIĆ, Stanislava KOŠKOVÁ, Jan VRBSKÝ, Petr DVOŘÁK a Albano MELI. Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process. In 2012 Mayo Young Investigators Research Symposium. 2012.

Základní údaje

• Originální název: Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process
• Název česky: DIFERENCIACE LIDSKÝCH EMBRYONÁLNÍCH KMENOVÝCH BUNĚK A LIDSKÝCH PLURIPOTENTNÍCH KMENOVÝCH BUNĚK V KARDIOMYOCYTY VE VYSOCE EFEKTIVNÍM PROCESU
• Název anglicky: Differentiation of human induced pluripotent and embryonic stem cells into cardiomyocytes in high yield process
• Autoři: PEŠL, Martin, Ivana AĆIMOVIĆ, Stanislava KOŠKOVÁ, Jan VRBSKÝ, Petr DVOŘÁK a Albano MELI.
• Vydání: 2012 Mayo Young Investigators Research Symposium, 2012.

Další údaje

• Typ výsledku: Konferenční abstrakt
• Utajení: není předmětem státního či obchodního tajemství
• Příznaky: Mezinárodní význam

Anotace

Rationale: Human induced pluripotent and embryonic stem cells (hiPS and hES) can be differentiated into cardiomyocytes (CMs) in vitro. Developed methods like cocultivation with END2 cells, differentiation on matrigel and also cytokine effect on clusters of cells, so called EB method. The current studies are facing very low yield and process variation according to the type of used stem cell line. Objective: The aim of this work is to generate human CMs from AggreWell plates through EB method. Methods and results: Cardiomyocytes were generated through modified protocol inspired by Gordon Keller s lab work(Toronto, Can). Subsequent analyzes of gene expression, imunohistochemistry and electrophysiology proved cardiomyocyte – like character of differentiated cells, neverthless the cells represent very young stage of developing heart and has to be further investigated. This basic research opens way for further pacient specific investigation on the level of cellular cardiology.

Anotace anglicky

Rationale: Human induced pluripotent and embryonic stem cells (hiPS and hES) can be differentiated into cardiomyocytes (CMs) in vitro. Developed methods like cocultivation with END2 cells, differentiation on matrigel and also cytokine effect on clusters of cells, so called EB method. The current studies are facing very low yield and process variation according to the type of used stem cell line. Objective: The aim of this work is to generate human CMs from AggreWell plates through EB method. Methods and results: Cardiomyocytes were generated through modified protocol inspired by Gordon Keller s lab work(Toronto, Can). Subsequent analyzes of gene expression, imunohistochemistry and electrophysiology proved cardiomyocyte – like character of differentiated cells, neverthless the cells represent very young stage of developing heart and has to be further investigated. This basic research opens way for further pacient specific investigation on the level of cellular cardiology.

Návaznosti

• MSM0021622430, záměr: Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie