CORE126 Modern technologies for life-science research

Pan-university studies
spring 2025
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Jiří Nováček, Ph.D. (lecturer)
Mgr. Alena Otoupalíková, Ph.D. (assistant)
doc. Mgr. Karel Kubíček, PhD. (lecturer)
MVDr. Boris Tichý, Ph.D. (lecturer)
Mgr. Vojtěch Bystrý, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
Mgr. Josef Houser, Ph.D. (lecturer)
doc. RNDr. Radovan Fiala, CSc. (lecturer)
doc. RNDr. Radka Svobodová, Ph.D. (lecturer)
Mgr. Jan Přibyl, Ph.D. (lecturer)
Mgr. Milan Ešner, Ph.D. (lecturer)
Ing. Michal Mikl, Ph.D. (lecturer)
Guaranteed by
Mgr. Jiří Nováček, Ph.D.
Central European Institute of Technology
Contact Person: Mgr. Alena Otoupalíková, Ph.D.
Supplier department: Cryo-Electron Microscopy and Tomography Core Facility – Centre for Structural Biology – Central European Institute of Technology
Timetable
Wed 26. 2. to Wed 21. 5. Wed 10:00–11:50 B11/335
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 120 student(s).
Current registration and enrolment status: enrolled: 4/120, only registered: 0/120, only registered with preference (fields directly associated with the programme): 0/120
Course objectives
The course will provide an introduction to understanding the structure of the basic building blocks of the cell and will cover the description of biophysical methods that can be used to study them.
Learning outcomes
Upon completion of the course, the student will be able to:
- explain the principles of modern technologies used in biological and biomedical research;
- understand basic principles of methods for studying the structure and function of biomolecules, including sequencing, proteomics, NMR spectroscopy, crystallography, and microscopy;
- understand basic principles of interpretation of experimental data obtained from different life science techniques;
- compare the advantages and limitations of different methods and select the appropriate approach for solving specific research problems;
- design experimental strategies for studying biological systems.
Syllabus
  • 1) 26.2.: Basic Building Blocks of the Cell (doc. Mgr. Karel Kubíček, Ph.D.)
  • 2) 5.3.: Principles of Methods for Studying Nucleic Acid Sequences (MVDr. Boris Tichý, Ph.D.)
  • 3) 12.3.: Analysis of Sequencing Data (Mgr. Vojtěch Bystrý, Ph.D.)
  • 4) 19.3.: Adventures in Proteomics (prof. RNDr. Zbyněk Zdráhal, Dr.)
  • 5) 26.3.: Biophysical Methods for Studying Structures and Interactions of Proteins and Nucleic Acids (Mgr. Josef Houser, Ph.D.)
  • 6) 2.4.: Principles for Studying the Structure and Dynamics of Proteins and Nucleic Acids Using NMR Spectroscopy (doc. RNDr. Radovan Fiala, CSc.)
  • 7) 9.4.: Determination of Protein and Macromolecular Structures by X-ray Crystallography (Mgr. Josef Houser, Ph.D.)
  • 8) 16.4.: Principles of Electron Microscopy and Its Applications in Biology (Mgr. Jiří Nováček, Ph.D.)
  • 9) 23.4.: In Silico Methods for Determining Biomolecular Structures (doc. RNDr. Radka Svobodová, Ph.D.)
  • 10) 30.4.: Atomic Force Microscopy (Mgr. Jan Přibyl, Ph.D.)
  • 11) 7.5.: Light Microscopy (Mgr. Milan Ešner, Ph.D.)
  • 12) 14.5. Excursion to the Laboratory
  • 13) 21.5.: Principles of MRI and Its Applications in Neuroimaging (Ing. Michal Mikl, Ph.D.)
Teaching methods
lectures, class discussion
Assessment methods
The course will be concluded with a colloquium, which can take one of two forms: either a discussion on a selected topic or the submission of a seminar thesis focusing on one or more methods introduced in the course.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/cus/spring2025/CORE126