ZLLM0421c Medical Oral Microbiology I - practice

Faculty of Medicine
spring 2020
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková, Ph.D. (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
Mgr. Ing. Veronika Korecká (seminar tutor)
Mgr. Katarína Rebrošová (seminar tutor)
doc. MUDr. Filip Růžička, Ph.D. (seminar tutor)
Mgr. Lukáš Vacek (seminar tutor)
doc. MUDr. Vladana Woznicová, Ph.D. (seminar tutor)
Guaranteed by
doc. MUDr. Filip Růžička, Ph.D.
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Timetable of Seminar Groups
ZLLM0421c/Dostud: No timetable has been entered into IS., Skupina pro dostudovávající ZL plus další studenty, kteří již mají VLLM0421c
ZLLM0421c/21_23: Thu 11:00–12:40 N02112, O. Zahradníček
ZLLM0421c/22_24: Thu 18:00–19:40 N02112, L. Černohorská
Prerequisites
ZLLC011p Medical Chemistry,DM - lecture && ZLBI0222p Biology II-lect. && ZLBF011p Med.Physics and Informat.-lect
Basic knowledge of biology, anatomy, physiology and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to understand basic methods of medical and medical oral microbiology.
Learning outcomes
At the end of this course, students should be able:
to prepare wet mount, Gram stained preparation, to observe the results in a microscope, to list other staining methods
to list culture media, their use, differences between them, to reinoculate a strain/specimen to a culture medium
to identify bacteria and yeasts by means of various methods of biochemical identification and other identification methods
to describe antibiotic susceptibility/resistance testing methods and to read their results
to describe animal experiment (only basically)
to read results of nucleic acid detection in microbiology (only basically)
to describe methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; to read the results of these methods including titers, titer dynamics, detection of IgM/IgA/IgG; to interprete the results; to describe precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will be able to describe the basic of medical virology
Syllabus
  • J01 Safety in laboratory, technique of work with loop, wet mount. Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms and structures in Gram staining.
  • J02 Bacterial culture
  • J03 Fysiology of bacteria, idenctification according to biochemical activity and virulence factor determination
  • J04 Microbes and outer influences, decontamination methods
  • J05 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J06 Introduction to serology, agglutination and precipitation, dilution and following titre dynamics
  • J07 Complementfixing test
  • J08 Neutralisatioin (ASO, HIT, VNT)
  • J09 Reactions with labelled components (IMF, RIA, ELISA) including immunoblotting
  • J10 Medical virology I (influenza, respiratory viruses, tick-borne encefalitis)
  • J11 Medical virology II (hepatitis and HIV))
  • J12 Medical parasitology (basics)
  • J13 Medical mycology (basics)
  • J14 Biofilm infections
  • J15 Review
Literature
Teaching methods
practical training
self-study with help of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- two justified absences or absences approved by teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed (EXTRA PAYMENT IS REQUIRED FOR EVENTUAL SUBSTITUTION!!!)
(2) complete laboratory report (signature of teacher is not necessary)
(3) succesfully written final test, eventually more tests; date of all tests will be declared in time.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
Teacher's information
http://www.medmicro.info
The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019.
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