aZLLM0421c Medical Oral Microbiology I - practice

Faculty of Medicine
spring 2023
Extent and Intensity
0/2/0. 1 credit(s). Type of Completion: z (credit).
Taught in person.
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková, Ph.D. (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
prof. MUDr. Filip Růžička, Ph.D. (seminar tutor)
doc. MUDr. Vladana Woznicová, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
aZLLC011p Med Chem, Dental Mat - lect && aZLBI0222p Biology II-lect. && aZLBF011p Med.Physics and Informat.-lect
Students are supposed to have considerable knowledge of anatomy, histology, biology, biochemistry and physiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, the students are supposed to understand methods used in medical microbiology, using medical oral microbiology.
Learning outcomes
At the end of this course, students should be able:
to prepare a wet mount, Gram-stained preparation, to observe the results in a microscope, to list other staining methods
to list culture media, their use, differences between them, to reinoculate a strain/specimen to a culture medium
to identify bacteria and yeasts by means of various methods of biochemical identification and other identification methods
to describe antibiotic susceptibility/resistance testing methods and to read their results
to describe animal experiment (only basically)
to read results of nucleic acid detection in microbiology (only basically)
to describe methods detecting antigens or antibodies, their use for antibody detection/antigen detection in specimen/antigen analysis of a strain; to read the results of these methods including titers, titer dynamics, detection of IgM/IgA/IgG; to interpret the results; to describe precipitation, agglutination, agglutination on carriers, complement-fixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will be able to describe the basis of medical virology
  • J01 Safety in the laboratory, the technique of work with loop, wet mount. Gram staining, demonstration of more staining methods. Demonstration of pictures of various organisms and structures in Gram staining.
  • J02 Bacterial culture
  • J03 Physiology of bacteria, identification according to biochemical activity and virulence factor determination
  • J04 Microbes and outer influences, decontamination methods
  • J05 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J06 Introduction to serology, agglutination and precipitation, dilution and measuring titres
  • J07 Following dynamics of titres, complement-fixing test, neutralisation
  • J08 Reactions with labeled components (IMF, RIA, ELISA) including immunoblotting
  • J09 Molecular methods in microbiology
  • J10 Medical virology I (influenza, respiratory viruses, tick-borne encephalitis)
  • J11 Medical virology II (hepatitis and HIV))
  • J12 Medical parasitology (basics)
  • J13 Medical mycology (basics)
  • J14 Biofilm infections, oral microbiology
  • J15 No practical sessions (only exceptionally individual substitutions)
    required literature
  • SAMARANAYKE, L.P. Essential Microbiology for Dentistry. 3rd Ed. Churchill Livingstone, 2006. 372 pp. ISBN 978-0-323-04475-2. info
    recommended literature
  • MARSH, Philip. Oral Microbiology. Wright, 2002. ISBN 0-7236-1051-7. info
  • BJARNSHOLT, Thomas, Claus MOSER and Niels HØIBY. Biofilm Infections. Springer, 2011. ISBN 978-1-4419-6084-9. e-book info
    not specified
  • Book No 1 + lectures + all materials from practical sessions are basic for the examination. Other books are recommended
Teaching methods
practical training in lab
self-study with use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with the following notes:
- two justified absences or absences approved by a teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed (EXTRA PAYMENT IS REQUIRED FOR EVENTUAL SUBSTITUTION!!!)
(2) complete laboratory report (signature of a teacher is not necessary)
(3) successfully written final test (usually 10 multiple choice questions, each for one point; 7 points needed)
(4) all ROPOT questionnaires completed successfully; if they are always fulfilled in time (prior to particular lab session), the student has a bonus of one point for the final test
Language of instruction
Further comments (probably available only in Czech)
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
The course is taught: every week.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022.
  • Enrolment Statistics (spring 2023, recent)
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