aVLLM0421c Medical Microbiology I - practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková, Ph.D. (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
Mgr. Ing. Veronika Korecká (seminar tutor)
Mgr. Katarína Rebrošová (seminar tutor)
prof. MUDr. Filip Růžička, Ph.D. (seminar tutor)
MUDr. Alena Siváková (seminar tutor)
Mgr. Lukáš Vacek (seminar tutor)
Guaranteed by
prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Timetable of Seminar Groups
aVLLM0421c/28_29: Mon 9:30–12:00 N02112, O. Zahradníček
aVLLM0421c/30_31: Tue 7:30–10:00 N02112, O. Zahradníček
aVLLM0421c/32_33: Tue 15:00–17:30 N02112, O. Zahradníček
aVLLM0421c/34_35: Tue 17:30–20:00 N02112, A. Siváková
aVLLM0421c/36_37: Fri 14:30–17:00 N02112, O. Zahradníček
aVLLM0421c/38_39: Thu 15:00–17:30 N02112, O. Zahradníček
aVLBI0222p Medical Biology II - lect. && aVLBF011p Biophysics -lect.
Students are supposed to have considerable knowledge of anatomy, histology, biology, biochemistry and physiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to understand the main diagnostic methods in medical microbiology.
Learning outcomes
At the end of this course, students should be able:
to prepare a wet mount, Gram-stained preparation, to observe the results in a microscope, to list other staining methods
to list culture media, their use, differences between them, to reinoculate a strain/specimen to a culture medium
to identify bacteria and yeasts by means of various methods of biochemical identification and other identification methods
to describe antibiotic susceptibility/resistance testing methods and to read their results
to describe animal experiment (only basically)
to read results of nucleic acid detection in microbiology (only basically)
to describe methods detecting antigens or antibodies, their use for antibody detection/antigen detection in specimen/antigen analysis of a strain; to read the results of these methods including titers, titer dynamics, detection of IgM/IgA/IgG; to interpret the results; to describe precipitation, agglutination, agglutination on carriers, complement-fixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will be able to describe the basis of medical virology
  • J01 Safety in a laboratory, technique of work with loop, wet mount. Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms and structures in Gram staining.
  • J02 Bacterial culture
  • J03 Physiology of bacteria, identification according to biochemical activity and virulence factor determination
  • J04 Molecular methods in microbiology
  • J05 Microbes and outer influences, decontamination methods
  • J06 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J07 Biofilm infections
  • J08 Introduction to serology, agglutination and precipitation, dilution and measuring titres
  • J09 Following dynamics of titres, complement-fixing test, neutralisation
  • J10 Reactions with labeled components (IMF, RIA, ELISA) including immunoblotting
  • J11 Medical virology I (hepatitis, HIV, herpesviruses)
  • J12 Medical virology II (influenza, respiratory viruses, tick-borne encephalitis)
  • J13 Medical parasitology (basics)
  • J14 Medical mycology (basics)
  • J15 No practical sessions (only exceptionally individual substitutions)
    required literature
  • Medical microbiology : a guide to microbial infections : pathogenesis, immunity, laboratory diagnosis and control. Edited by David Greenwood. 18th ed. Edinburgh: Churchill Livingstone, 2012. xvi, 778. ISBN 9780702040900. info
    recommended literature
  • Mims' medical microbiology. Edited by Richard V. Goering - Cedric A. Mims. 4th ed. Philadelphia, PA: Mosby Elsevier, 2008. xi, 656. ISBN 9780808923725. info
  • Manual of clinical microbiology. Edited by James Versalovic. 10th ed. Washington: ASM Press, 2011. xxiii, 126. ISBN 9781555814632. info
  • BJARNSHOLT, Thomas, Claus MOSER and Niels HØIBY. Biofilm Infections. Springer, 2011. ISBN 978-1-4419-6084-9. e-book info
    not specified
  • Book No 1 + lectures + all materials from practical sessions are basic for the examination. Other books are recommended.
Teaching methods
practical training in lab
self-study with the use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with the following notes:
- no substitution is needed for one justified absence + one without official justification
- in case of more justified absences, some form of substitution (e. g. some homework) is needed
- not justified absences (except that one "sick day") are not allowed
(2) complete laboratory report (signature of a teacher is not necessary)
(3) successfully written final test (usually 10 multiple choice questions, each for one point; 7 points needed)
(4) all ROPOT questionnaires completed successfully; if they are always fulfilled in time (prior to particular lab session), the student has a bonus of one point for the final test
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2023.
  • Enrolment Statistics (recent)
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