aVLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. David Galuška (assistant)
Martina Hanousková (assistant)
PharmDr. Jiří Kos, Ph.D. (assistant)
Miriam Minaříková (assistant)
Bc. Ivana Ševčíková (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
aVLBC0422c/28: Thu 11:00–13:30 A16/212, J. Slanina
aVLBC0422c/29: Thu 11:00–13:30 A16/216, H. Paulová
aVLBC0422c/30: Thu 11:00–13:30 A16/211, M. Králíková
aVLBC0422c/31: Tue 11:00–13:30 A16/211, O. Peš
aVLBC0422c/32: Tue 8:00–10:30 A16/211, M. Tomandlová
aVLBC0422c/33: Tue 8:00–10:30 A16/212, H. Paulová
aVLBC0422c/34: Wed 11:00–13:30 A16/211, J. Smutná
aVLBC0422c/35: Wed 11:00–13:30 A16/216, J. Gregorová
aVLBC0422c/36: Tue 11:00–13:30 A16/216, H. Paulová
aVLBC0422c/37: Wed 11:00–13:30 A16/212, O. Peš
aVLBC0422c/38: Tue 11:00–13:30 A16/212, J. Slanina
aVLBC0422c/39: Tue 8:00–10:30 A16/216, J. Slanina
Prerequisites (in Czech)
aVLBC0321s Biochemistry I - seminar && aVLFY0321c Physiology I - practice && aVLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to aVLBC0422p. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
The student is able:
- to explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation.
- to describe physiological values of the most important biochemical parameters.
- to interpret the results of biochemical analyzes, discusses the significance of the findings.
- to understand the principles of the most important biochemical-analytical methodologies.
- to be oriented in basic laboratory manipulations.
  • Organization of work in practical exercises. Handling with biological material.
  • Blood collection and processing.
  • Pipetting.
  • Communication of biochemical test results.
  • Determination of plasma glucose, determination of glycemia by personal glucometer. Oral glucose tolerance test (oGTT). Evidence of glucosuria and ketonuria. Determination of glycated hemoglobin.
  • Determination of total cholesterol in serum and blood. Determination of triacylglycerols in blood. Determination of HDL- and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. Determination of catalytic concentrations of aminotransferases in serum.
  • Determination of total serum bilirubin. Detection of bilirubin and urobilinogens in urine.
  • Determination of serum albumin.
  • Determination of total protein and serum albumin. Serum protein electrophoresis.
  • Detection and determination of C-reactive protein in serum and blood. Determination of ferritin in serum.
  • Objective and physical examination of urine.
  • Determination of creatinine in serum and urine. Determination of glomerular filtration from creatinine clearance. Calculation of fractional water resorption and excretion.
  • Proof and determination of proteinuria and albuminuria. Evidence of blood pigment in the urine. Detection of Bence-Jones protein in urine.
  • Determination of urea in serum and urine. Nitrogen balance.
  • Examination of urine with multifunctional diagnostic strips. Demonstration of drugs in urine. Detection of luteinizing hormone in urine.
  • Solubility of uric acid. Orientational chemical analysis of urinary stones.
  • Determination of uric acid in serum and urine.
  • Selected laboratory examinations of the stomach. Laboratory tests for pancreatic diseases Digestive tract occult bleeding test.
    required literature
  • Instructions available in IS MUNI.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI,
  • successful completion of the credit test (at least 70% of points),
  • course-unit credit from aZC041.
    The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
  • Language of instruction
    Further comments (probably available only in Czech)
    The course is taught annually.
    General note: Successful completion of the course is a condition for access to the exam in the course aVLBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    Successful completion of the course is a condition for access to the exam in the course aVLBC0422p.
    The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021.
    • Enrolment Statistics (recent)
    • Permalink: https://is.muni.cz/course/med/spring2022/aVLBC0422c