aZLBC0422c Biochemistry II -practice

Faculty of Medicine
spring 2021
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Taught in person.
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Martina Hanousková (assistant)
Miriam Minaříková (assistant)
Mgr. Jindra Smutná, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Wed 10. 3. 8:00–9:40 A16/212, 8:00–9:40 A16/216, Wed 31. 3. 8:00–9:40 A16/216, 8:00–9:40 A16/212, Wed 21. 4. 8:00–9:40 A16/212, 8:00–9:40 A16/216
Prerequisites (in Czech)
aZLLC011p Medical Chemistry,DM - lecture && aZLBF011p Med.Physics and Informat.-lect && aZLBI0222p Biology II-lect. && ( aZLBC0221s Biochemistry I - seminar || aZLBC0321s Biochemistry I - seminar )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is complementary to aZLBC041p and aZLBC041s. The aim of the course is to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics
Learning outcomes
Upon completion of the course the student will be able to:
- manipulate with basic laboratory equipment and respect the principles of safe work in the laboratory
- explain how to obtain biological material for biochemical analysis and respects the rules of manipulation.
- understand the principles of interpretation of biochemical analyzes results, discusses the significance of test results.
- understand the principles of the most important biochemical-analytical methodologies.
  • 1. Directions for laboratory work. Investigation of glucose metabolism Investigation of glucose metabolism Enzymatic determination of plasma glucose Determination of glycaemia by personal glucometer Test for glucose in urine. Test for ketone bodies in urine ---- 2. Investigation of hard tissues Determination of calcium in serum Determination of total ALP catalytic concentration and bone ALP isoform catalytic concentration. --- 3. Investigation of saliva Investigation of salivary secretion rate Estimation of salivary buffering capacity Detection of selected organic compounds in saliva Detection of amylase in saliva and its substrate specificity
    required literature
  • Instructions available in IS.
Teaching methods
laboratory practicals
Assessment methods
Conditions of giving the credits • Submission of all completed protocols to the IS Homework Vaults in relevant dates. • Required attendance in on-line classes. • All absences must be made up according to teacher instructions. • All conditions must be fulfilled till 15. 6. 2021. Obtaining of course-unit credits of practical classes and seminars is the pre-requisite for registration to the examination of Biochemistry II.
Language of instruction
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 15.
Information on course enrolment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu
Listed among pre-requisites of other courses

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