aZLBC0422c Biochemistry II -practice

Faculty of Medicine
spring 2023
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Taught in person.
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Prerequisites (in Czech)
aZLLC011p Med Chem, Dental Mat - lect && aZLBF011p Med.Physics and Informat.-lect && aZLBI0222p Biology II-lect. && ( aZLBC0221s Biochemistry I - seminar || aZLBC0321s Biochemistry I - seminar )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is complementary to aZLBC0422p. The aim of the course is to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics
Learning outcomes
Upon completion of the course the student will be able to:
- manipulate with basic laboratory equipment and respect the principles of safe work in the laboratory
- explain how to obtain biological material for biochemical analysis and respects the rules of manipulation.
- understand the principles of interpretation of biochemical analyzes results, discusses the significance of test results.
- understand the principles of the most important biochemical-analytical methodologies.
  • Organization of work in practical exercises.
  • Enzymatic determination of plasma glucose. Determination of glycemia by personal glucometer. Detection and determination of glucose in urine. Evidence of urinary ketone bodies.
  • Determination of calcium in serum. Determination of the catalytic activity of total ALP and bone isoenzyme in serum.
  • Examination of saliva secretion rate. Determination of saliva buffering capacity. Identification of selected organic substances in saliva. Detection of amylase in saliva and its substrate specificity.
    required literature
  • Instructions available in IS MUNI.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all lessons,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI.
    The detailed conditions are posted in Information System (Study materials – Course-related instructions).
  • Language of instruction
    Further comments (probably available only in Czech)
    The course is taught annually.
    The course is taught: every week.
    General note: Successful completion of the course is a condition for access to the exam in the course aZLBC0422p.
    Information on the extent and intensity of the course: 15.
    Information on course enrolment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu
    Listed among pre-requisites of other courses

    Zobrazit další předměty

    Teacher's information
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required for the exercise.
    Consultations are possible after a personal arrangement with the teacher.
    Successful completion of the course is a condition for access to the exam in the course aZLBC0422p.
    The course is also listed under the following terms spring 2021, spring 2022.
    • Enrolment Statistics (spring 2023, recent)
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