aVLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2019
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Ing. Martina Čarnecká, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
PharmDr. Katarína Kostolanská (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Roman Sándor (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Martina Hanousková (assistant)
Miriam Minaříková (assistant)
Lenka Nerudová (assistant)
Mgr. Lucie Novotná (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
aVLBC0422c/30: Tue 8:00–10:30 A16/212, M. Hlaváčová
aVLBC0422c/31: Tue 8:00–10:30 A16/211
aVLBC0422c/32: Thu 11:00–13:30 A16/212
aVLBC0422c/33: Fri 8:00–10:30 A16/216, J. Tomandl
aVLBC0422c/34: Mon 11:00–13:30 A16/212
aVLBC0422c/35: Thu 14:00–16:30 A16/212
aVLBC0422c/36: Thu 11:00–13:30 A16/211
aVLBC0422c/37: Fri 8:00–10:30 A16/211
aVLBC0422c/38: Mon 11:00–13:30 A16/211, M. Králíková
Prerequisites (in Czech)
aVLBI0222p Biology II - lect. && aVLBF011p Biophysics -lect. && aVLBC0321s Biochemistry I - sem. && aVLBC0321c Biochemistry I - pract. && aVLFY0321c Physiology I - practice && aVLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to aVLBC0421p and aVLLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
The student is able:
- to explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation.
- to describe physiological values of the most important biochemical parameters.
- to interpret the results of biochemical analyzes, discusses the significance of the findings.
- to understand the principles of the most important biochemical-analytical methodologies.
- to be oriented in basic laboratory manipulations.
  • • Directions for laboratory work. Handling biological material. Laboratory examination. Blood, plasma, serum. Blood collection. Blood processing. Manual methods, pipetting. Biochemical analyzers. Test requisition forms. • Investigations of glucose metabolism. Diabetes mellitus, Enzymatic determination of plasma glucose. Determination of glycaemia by personal glucometer. Oral glucose tolerance test (oGTT). Detection and determination of glucose in urine. Detection of ketone bodies in urine. Determination of glycated hemoglobin (HbA1c). • Investigation of lipid and cholesterol metabolism. Enzymatic determination of serum total cholesterol. Determination of blood cholesterol using Accutrend. Determination of blood triacylglycerols using Accutrend. Determination of HDL-cholesterol and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. • Investigations in liver disease I – Enzymes. Determination of catalytic concentrations of aminotranferases in serum. Determination of ALP catalytic concentration in serum. • Selected investigations in liver disease II – Bile pigments and albumin. Determination of total bilirubin in serum. Detection of bilirubin in urine. Detection of urobilinogens in urine. Determination of serum albumin. • Investigation of plasma proteins. Determination of total protein by the biuret test. Determination of albumin in serum. Electrophoresis of serum proteins. Assay for increased level of C-reactive protein. Determination of serum IgE by ELISA method. • Biochemical tests of renal function – I. Investigation of urine by inspection and physical methods. Determination of creatinine in serum and urine. Estimation of glomerular filtration rate from creatinine clearance. Fractional reabsorption and excretion of water. • Biochemical tests of renal function – II. Detection of proteinuria. Urine test for haem compounds. Determination of protein concentration in urine. Detection of microalbuminuria. Detection of Bence Jones protein in urine. Determination of urea in serum and urine. Approximate assessment of the nitrogen balance. • Routine urinalysis. Test strips. Multipurpose diagnostic strips in urinalysis. Detection of addictive drugs in urine. Detection of barbiturates in urine. Detection of luteinizing hormone in urine. • Examinations in urolithiasis. Solubility of uric acid. Detection of uric acid by murexide reaction. Simplified chemical analysis of renal calculi. Determination of uric acid in serum and urine. • Some investigations of the gastrointestinal tract. Selected examinations of stomach functions. Laboratory tests in pancreas diseases. Test of occult bleeding in gastrointestinal tract.
  • Texts available in Information System.
Teaching methods
laboratory practicals
Assessment methods
Full attendance in all practicals is the condition for obtaining of course-unit credits.
Language of instruction
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2017, Spring 2018, spring 2020, spring 2021.
  • Enrolment Statistics (spring 2019, recent)
  • Permalink: https://is.muni.cz/course/med/spring2019/aVLBC0422c