BLHM0321c Histopathological Techniques I

Faculty of Medicine
Autumn 2008
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Drahomír Horký, DrSc. (lecturer)
doc. MUDr. Miroslava Sedláčková, CSc. (lecturer)
doc. MUDr. Jitka Šťastná, CSc. (lecturer)
Jana Puklová (assistant)
Guaranteed by
doc. MUDr. Miroslava Sedláčková, CSc.
Department of Histology and Embryology - Theoretical Departments - Faculty of Medicine
Contact Person: doc. MUDr. Miroslava Sedláčková, CSc.
Timetable of Seminar Groups
BLHM0321c/01: each even Tuesday 9:00–12:20 F01B1/223
BLHM0321c/02: each odd Tuesday 9:00–12:20 F01B1/223
BLHM0321c/03: each even Monday 9:00–12:20 F01B1/223
Prerequisites (in Czech)
BLHV0211c Histopatologické metody - cvičení
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to provide students with methods of tissue processing for light and electron microscopy for histopathological diagnostic purposes. Students will obtain practical skills in tissue fixation, embedding, cutting and staining and will be able to prepare histologic slides stained according to the specific requests.
Syllabus
  • Equipment of histologic laboratory. Safety in the histologic laboratory with special respect to work with microtomes and chemicals.
  • Taking of material for histologic examination. Fixation, physical and chemical methods of fixation, advantage and disadvantage of both methods. Principal types of fixatives - formaldehyde, picric acid, mercuric chloride, other fixatives.
  • Principles of tissue embedding into paraffin wax or paraplast. Practical implementation of dehydration, clearing, and embedding.
  • Embedding of hard tissues into celloidin, embedding into water soluble media.
  • Cutting of tissue blocks, sliding and rotary microtomes, freezing microtome, cryostat.
  • Cutting of tissue blocks on sliding microtome. Stretching and sticking of sections. Common faults.
  • Cutting of tissue blocks on rotary microtome: successive sections.
  • Staining of sections. Types of histologic staining dyes. Preparation of staining solutions.
  • Hematoxylin-eosin staining.
  • Other staining methods. AZAN, impregnation. Weigert - van Gieson staining.
  • Histopathologic methods, staining of amyloid.
  • Evidence of inorganic substances. Evidence of some pigments - hemoglobin, melanin.
  • Mallory staining.
  • Basics of immunohistochemistry.
  • Evidence of polysaccharides - evidence of glycogen by PAS reaction and Best carmine.
  • Preparation of hard tissues.
  • Evidence of lipids by lipid soluble staining dyes.
  • Tissue processing for electron microscopy.
Literature
  • VACEK, Zdeněk. Histologie a histologická technika. 1. vyd. Brno: Institut pro další vzdělávání pracovníků ve zdravotnictví, 1995. 184 s. ISBN 80-7013-202-7. info
  • Theory and practice of histological techniques. Edited by John D. Bancroft - Marilyn Gamble. 5th ed. Edinburgh: Churchill Livingstone, 2003. xiii, 796. ISBN 0443064350. info
Assessment methods
Practical implementation of tissue processing in histologic laboratory. Giving the course-unit credit is conditioned by full attendance in the lessons. Adequate theoretical knowledge will be tested.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2008/BLHM0321c