aFAAI1_15 Analytical Chemistry for Pharmacists I

Faculty of Pharmacy
Autumn 2025

The course is not taught in Autumn 2025

Extent and Intensity
2/3/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (lecturer)
Mgr. Tomáš Crha (seminar tutor)
Mgr. Michaela Kuchynka, Ph.D. (seminar tutor)
Ing. Klára Odehnalová, Ph.D. (seminar tutor)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (seminar tutor)
Mgr. Hana Pížová, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Bc. Jiří Pazourek, Ph.D.
Department of Chemical Drugs – Departments – Faculty of Pharmacy
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Abstract
Objectives: To provide the theoretical and practical knowledge for qualitatively performing chemical and instrumental analysis of inorganic and organic substances including pharmaceuticals, emphasizing the importance of methods e.g. in drug analysis and in studying structural and physico-chemical properties of substances related to their biological effect. Emphasis is placed on shaping the logical thought processes of a pharmacist important for his future professional use. Practical exercises are required to master working habits and students' independent approach to solving analytical problems.
Learning outcomes
After completing the course, the student will be able to:
- understand the meaning and principles of classical and modern analytical chemistry
- have an overview of methods of qualitative analytical chemistry
- practically perform basic laboratory tests of qualitative analytical chemistry
Key topics
  • Content of the lectures 1.-2. Analytical chemistry. The goal and basic terms of the science, methods. Analytical reactions. Protolytic, redox, precipitation and complexation reactions. Buffers.
  • 3.-6. Qualitative analysis of inorganic compounds. Group reactions of cations and anions. Selective and specific reactions of common ions.
  • 7.-8. Qualitative analysis of organic compounds - Identification of organic compounds. Elemental analysis. Classical confirmations and identification of functional groups. Preliminary test. Determination of basic physical constants. Classes of solubility.
  • 9. Qualitative analysis of organic compounds. Instrumental methods of structure analysis: UV-VIS, interpretation of the spectra, examples.
  • 10. Qualitative analysis of organic compounds. Instrumental methods of structure analysis: IR, interpretation of the spectra, examples.
  • 11. Qualitative analysis of organic compounds. Instrumental methods of structure analysis: NMR, interpretation of the spectra, examples.
  • 12. Qualitative analysis of organic compounds. Instrumental methods of structure analysis: MS, interpretation of the spectra, examples.
  • MOODLE grade test (60 min). Content of the laboratory exercises 1.+2. Organization of the exercises. Safety rules for a chemical laboratory. Basic operations in the analytical laboratory. Good laboratory practice (GLP). Group reactions of cations - group reagent: HCl, H2SO4, (COOH)2, (NH4)2S,
    NaOH, NH3, KI, CH3COONa. Analysis of an unknown sample.
  • 3.+4. Preliminary test. Group reactions of anions, group reagents: BaCl2, AgNO3, KMnO4, I2, KI. Analysis of an unknown sample. 5.+6. Preliminary test. Analysis of organic compounds, preliminary tests, elemental analysis, determination of the class of solubility. Analysis of two unknown samples.
  • 7.+8. Preliminary test. Physical properties of compounds: measurement of melting point, refractive index, pH. Calculation of analytical concentration of a weak acid from a pH measurement. 9.+10. Preliminary test. Identification of organic compounds from UV-vis spectra. Influence of pH on the absorption spectrum of an acid-base indicator.
  • 11.+12. Preliminary test. Identification of compounds by Infrared spectrometry (IR). Comparison of KBr-tablet technique (transmission mode) and Attenuated total reflection (ATR) technique. Analysis of an unknown sample.
Study resources and literature
    recommended literature
  • European Pharmacopoeia
  • WATSON, David G. and Bhavik A. PATEL. Pharmaceutical analysis : a textbook for pharmacy students and pharmaceutical chemists. Fifth edition. Edinburgh: Elsevier, 2021, vi, 462. ISBN 978070208088. info
  • HAM, Bryan M. and Aihui MAHAM. Analytical chemistry : a chemist and laboratory technician's toolkit. Hoboken, New Jersey: John Wiley & Sons, 2016, xxiv, 652. ISBN 9781118714843. info
  • CHRISTIAN, Gary D.; Purnendu K. DASGUPTA and Kevin SCHUG. Analytical chemistry. 7th ed. Hoboken, N.J.: Wiley, 2014, xxii, 826. ISBN 9780470887578. info
Approaches, practices, and methods used in teaching
Monologic (reading, lecture, demonstration) Laboratory work
Method of verifying learning outcomes and course completion requirements
written grade test; 51% of correct answers is needed to pass
Language of instruction
English
Teacher's information
http://is.muni.cz/auth/el/pharm/podzim2020/F1AI1_15/index.qwarp
To obtain credit a student must satisfactory complete all exercises, submit protocols from each exercise and successfuly pass a credit test. Topics of questions for the written credit test: Confirmation reactions of analytical chemistry. Group and selective reactions of cations and anions: examples of the most important reactions, reaction conditions. Elementary qualitative analysis, determination of basic physical constants and solubility classes of organic substances. Evidence of oxygen and nitrogen functional groups. Identification of organic substances by instrumental methods: UV-VIS, IR, NMR, MS. Solution of the structure according to spectra (or summary formula and other data).
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