FaF:F1BL2_16 Biotechnology of Drugs-practic - Course Information

F1BL2_16 Biotechnology of Drugs - practical classes

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Mgr. Marie Brázdová, Ph.D. (seminar tutor)
Mgr. Robert Helma, Ph.D. (seminar tutor)
prof. Ing. René Kizek, DrSc., MBA (seminar tutor)
Ing. Marcela Nejezchlebová (seminar tutor)
Mgr. Marek Petr, Ph.D. (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)

Guaranteed by

PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy

Prerequisites (in Czech)

3120/F1ME1 Molekulární biologie
3120/F1MG1 Metody molekulární biologie
3120/F1BL1 Biotechnologie léčiv

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Pharmacy (programme FaF, M-FARM)

Course objectives

This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.

Learning outcomes

Basic knowledge in methods of pharmaceutical biotechnology

Syllabus

• 1) Isolation of chromosomal DNA
  2) Amplification of selected gene by polymerase chain reaction
  3) Purification of PCR products
  4) Isolation of plasmid DNA
  5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
  6) Digestion of vector DNA by restriction endonucleases
  7) Ligation of PCR product into a vector
  8) Transformation of bacterial cells Escherichia coli
  9) PAGE

Literature

required literature
• Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
• Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info

not specified
• Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info

Teaching methods (in Czech)

Laborování

Assessment methods (in Czech)

Rozbor produktů pracovní činnosti studenta (technické práce)

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.

Teacher's information

100% attendance at compulsory exercises
laboratory protocols
final test

Activity	Difficulty [h]
Domácí příprava na výuku	5
Účast na výuce	30
Summary	35

• Enrolment Statistics (Spring 2020, recent)
  
• Permalink: https://is.muni.cz/course/pharm/spring2020/F1BL2_16