FaF:F1BL2_16 Biotechnology of Drugs-practic - Course Information

F1BL2_16 Biotechnology of Drugs - practical classes

Extent and Intensity

0/2/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Ing. Marcela Nejezchlebová (seminar tutor)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)

Guaranteed by

PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy

Timetable of Seminar Groups

F1BL2_16/01: each odd Wednesday 13:00–16:20 45-324, J. Treml
F1BL2_16/02: each odd Wednesday 16:20–19:40 45-324, D. Rotrekl
F1BL2_16/03: each even Wednesday 13:00–16:20 45-324, J. Treml
F1BL2_16/04: each even Wednesday 16:20–19:40 45-324, D. Rotrekl
F1BL2_16/05: each odd Thursday 13:00–16:20 45-324, M. Nejezchlebová
F1BL2_16/06: each even Thursday 13:00–16:20 45-324, M. Nejezchlebová

Prerequisites (in Czech)

3120/F1ME1 Molekulární biologie
3120/F1MG1 Metody molekulární biologie
3120/F1BL1 Biotechnologie léčiv

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 72 student(s).
Current registration and enrolment status: enrolled: 8/72, only registered: 0/72

fields of study / plans the course is directly associated with

• Pharmacy (programme FaF, M-FARM)

Course objectives

This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.

Learning outcomes

Basic knowledge in methods of pharmaceutical biotechnology

Syllabus

• 1) Isolation of chromosomal DNA
  2) Amplification of selected gene by polymerase chain reaction
  3) Purification of PCR products
  4) Isolation of plasmid DNA
  5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
  6) Digestion of vector DNA by restriction endonucleases
  7) Ligation of PCR product into a vector
  8) Transformation of bacterial cells Escherichia coli
  9) PAGE

Literature

required literature
• Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
• Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info

not specified
• Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info

Teaching methods

Practical work in laboratory. Students create their own protocols.

Assessment methods

100% attendance in classes, 3 protocols and 60% points in credit test.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.

Teacher's information

100% attendance at compulsory exercises
laboratory protocols
final test

Activity	Difficulty [h]
Domácí příprava na výuku	5
Účast na výuce	30
Summary	35

• Enrolment Statistics (Spring 2022, recent)
  
• Permalink: https://is.muni.cz/course/pharm/spring2022/F1BL2_16