F1BL2_16 Biotechnology of Drugs - practical classes

Faculty of Pharmacy
Spring 2021
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
Ing. Marcela Nejezchlebová (seminar tutor)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable of Seminar Groups
F1BL2_16/01: each odd Wednesday 11:45–14:45 45-324, J. Treml
F1BL2_16/02: each odd Wednesday 14:45–17:45 45-324, D. Rotrekl
F1BL2_16/03: each odd Thursday 12:30–15:30 45-324, M. Nejezchlebová, D. Nykodýmová
F1BL2_16/04: each even Wednesday 11:45–14:45 45-324, J. Treml
F1BL2_16/05: each even Wednesday 14:45–17:45 45-324, D. Rotrekl
F1BL2_16/06: each even Thursday 12:30–15:30 45-324, M. Nejezchlebová, D. Nykodýmová
Prerequisites (in Czech)
3120/F1ME1 Molekulární biologie
3120/F1MG1 Metody molekulární biologie
3120/F1BL1 Biotechnologie léčiv
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 65 student(s).
Current registration and enrolment status: enrolled: 2/65, only registered: 0/65
fields of study / plans the course is directly associated with
Course objectives
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Learning outcomes
Basic knowledge in methods of pharmaceutical biotechnology
Syllabus
  • 1) Isolation of chromosomal DNA
    2) Amplification of selected gene by polymerase chain reaction
    3) Purification of PCR products
    4) Isolation of plasmid DNA
    5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
    6) Digestion of vector DNA by restriction endonucleases
    7) Ligation of PCR product into a vector
    8) Transformation of bacterial cells Escherichia coli
    9) PAGE
Literature
    required literature
  • Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
    not specified
  • Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
Teaching methods
Practical work in laboratory. Students create their own protocols.
Assessment methods
100% attendance in classes, 3 protocols and 60% points in credit test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
Teacher's information
100% attendance at compulsory exercises
laboratory protocols
final test
ActivityDifficulty [h]
Domácí příprava na výuku5
Účast na výuce30
Summary35
The course is also listed under the following terms Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2021, recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2021/F1BL2_16