FABL2_16 Biotechnology of Drugs - practical classes

Faculty of Pharmacy
Spring 2020
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Prerequisites
3120/FAME1 Molecular Biology I
3120/FAMG1 Methods of Molecular Biology
3120/FABL1 Biotechnology of Drugs
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Abstract
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Learning outcomes
Basic knowledge in methods of pharmaceutical biotechnology
Key topics
1) Isolation of plant chromosomal DNA
2) Amplification of selected plant gene by polymerase chain reaction
3) Purification of PCR products
4) Isolation of plasmid DNA
5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
6) Digestion of vector DNA by restriction endonucleases
7) Ligation of PCR product into a vector
8) Transformation of bacterial cells Escherichia coli
9) PAGE
Study resources and literature
    required literature
  • Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
    not specified
  • Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
Approaches, practices, and methods used in teaching (in Czech)
Laborování
Method of verifying learning outcomes and course completion requirements (in Czech)
Rozbor produktů pracovní činnosti studenta (technické práce)
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
100% attendance at compulsory exercises
laboratory protocols
final test
Activity Difficulty [h]
Domácí příprava na výuku 5
Účast na výuce 30
Summary 35
The course is also listed under the following terms Spring 2022, Spring 2024, Spring 2025, Spring 2026.
  • Enrolment Statistics (Spring 2020, recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2020/FABL2_16