FABL2_16 Biotechnology of Drugs - Practical Classes

Faculty of Pharmacy
Spring 2026
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
Mgr. et Mgr. Ondřej Pěnčík, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Contact Person: Mgr. Tereza Jůnová
Timetable of Seminar Groups
FABL2_16/01: each even Thursday 13:00–16:20 45-324
Prerequisites
NOW( FABL1_16 Biotechnology of Drugs ) && STUDIJNI_SKUPINA(start_before_2025) || PROGRAM(KOS)
The knowledge of subject in range of lectures.s
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 3/12, only registered: 0/12
fields of study / plans the course is directly associated with
Abstract
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Learning outcomes
Basic knowledge in methods of pharmaceutical biotechnology
Key topics
1) Isolation of plant chromosomal DNA
2) Amplification of selected plant gene by polymerase chain reaction
3) Purification of PCR products
4) Isolation of plasmid DNA
5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
6) Digestion of vector DNA by restriction endonucleases
7) Ligation of PCR product into a vector
8) Transformation of bacterial cells Escherichia coli
9) PAGE
Study resources and literature
    required literature
  • Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
    not specified
  • Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
Approaches, practices, and methods used in teaching
LAabs
Method of verifying learning outcomes and course completion requirements
Analysis of student work products (technical work)
Language of instruction
English
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is also listed under the following terms Spring 2020, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2026/FABL2_16