Bi4030c Molecular biology - practice

Faculty of Science
autumn 2021
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Tibor Botka, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Timetable of Seminar Groups
Bi4030c/01_1: Tue 8:00–13:50 D36/216, T. Botka
Bi4030c/01_2: Tue 8:00–13:50 D36/216, T. Botka
Bi4030c/02_1: Thu 13:00–18:50 D36/216, T. Botka
Bi4030c/02_2: Thu 13:00–18:50 D36/216, T. Botka
Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology )
Basic knowledge of general genetics, microbiology, inorganic chemistry, organic chemistry and biochemistry.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
This practical course is conceived as an introduction into methods of molecular biology. By the end of the course students should be able to analyze the genetic material including chromosomal DNA and perform cloning experiments with plasmids originating from bacteria.
Learning outcomes
Student will be able to:
isolate plasmid DNA of sufficient quality and quantity;
analyze isolated genetic material by basic methods of molecular biology such as restriction endonuclease cleavage and ELFO;
prepare sufficient amount of PCR amplicon of the gene of interest;
create the construct of the plasmid vector and the insert of the gene of interest and perform the transformation of competent cells;
use theoretical knowledge of other methods and principles in subsequent laboratory practice
  • 1. Preparation of solutions and stock enzymes used in molecular biology.
  • 2. Isolation of plasmid DNA (pUC series, pBluescript). Determination of DNA concentration and purity.
  • 3. Restriction analysis of nucleic acids, agarose gel electrophoresis.
  • 4. Restriction mapping, isolation of DNA from agarose gels.
  • 5. DNA cloning in basic types of vectors, DNA transfer to prokaryotic cells, transformation, selection of clones.
  • 6. Centrifugation techniques, preparation of saccharose density gradients.
  • 7. Isolation of bacterial genomic DNA for DNA fingerprinting.
  • 8. Polymerase chain reaction (PCR) and its modifications (PCR-RFLP, AP-PCR).
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The practical course is taught in seven 5-hour blocks in a molecular biology laboratory. Students perform the methods according to pre-examined protocols.
Assessment methods
The prerequisites for the successful course completion include participation in every practical exercise, elaboration of protocols out of the performed tasks and passing a written exam with 20 practically focused questions.
Language of instruction
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2019, Autumn 2020.
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