PřF:Bi4010c Essential mol. biol - seminar - Course Information
Bi4010c Essential molecular biology - seminarFaculty of Science
- Extent and Intensity
- 0/1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- ! Bi4020c Molecular biology - practice && ( NOW ( Bi4010 Essential molecular biology ) || NOW ( Bi4020 Molecular biology ) || Bi4010 Essential molecular biology || Bi4020 Molecular biology )
Basic knowledge of general genetics, physiology, anorganic chemistry, organic chemistry and biochemistry.
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 25 fields of study the course is directly associated with, display
- Course objectives
- This lecture is conceived as an introduction into molecular biology methods and it is focused on basic techniques commonly used for study of proteins, nucleic acids and their interactions in living cells and in vitro. It is recommended to all biology students who are not specialized in molecular biology. Understanding of principle and practical use of molecular biology methods is emphasized.
- Learning outcomes
- At the end of the course, students should be able to understand principles of the methods and make reasoned decisions about choosing the right methodological approaches to address specific biological phenomena.
- 1. Structure of nucleic acids - seminar (primary, secondary and tertiary structure of DNA and RNA, different conformations of DNA and their significance in biological systems).
- 2. Nucleic acids: manipulation, centrifugation, modifications in vitro.
- 3. Electrophoretic analyses of nucleic acids.
- 4. Hybridisation of nucleic acids: probe preparation, labelling.
- 5. Restriction analysis of nucleic acids, restriction map construction, genome mapping.
- 6. Sequence analyses of nucleic acids, genome sequencing.
- 7. DNA cloning (characterization and using of basic types of vectors, cloning strategies, DNA transfer to prokaryotic cells).
- 8. Polymerase chain reaction.
- 9. Basic methods of molecular diagnostics.
- 10. Basic methods of bioinformatics.
- BROWN, T. A. Gene cloning and DNA analysis : an introduction. 5th ed. Oxford: Blackwell Publishing, 2006. xx, 386. ISBN 1405111216. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005. 194 pp. 1. vydání. ISBN 80-210-3841-1. info
- ROSYPAL, Stanislav. Úvod do molekulární biologie. Třetí inovované vydání. Brno, 1998. 300 pp. Díl I. Informační makromolekuly. ISBN 80-902562-0-1. URL info
- Teaching methods
- Weekly lectures using powerpoint presentations and demonstration of selected techniques.
- Assessment methods
- Examination consists written test containing 30 questions.
- Language of instruction
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Listed among pre-requisites of other courses