Bi5137 Ancient protein analysis

Faculty of Science
Autumn 2025
Extent and Intensity
0/2/0. 3 credit(s). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Eva Chocholová, Ph.D. (seminar tutor)
prof. RNDr. Zbyněk Zdráhal, Dr. (seminar tutor)
doc. RNDr. Eva Drozdová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Eva Drozdová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Eva Chocholová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science (50,00 %), Zbyněk Zdráhal Research Group – Mendel Centre for Plant Genomics and Proteomics – Central European Institute of Technology (50,00 %)
Prerequisites (in Czech)
Bi6129 Palaeo - genomics, proteomics && Bi4020 Molecular biology &&3580
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to introduce students to the field of palaeoproteomics in more detail and to provide an opportunity for practical application of knowledge in block classes.
Learning outcomes
Upon completion of the course, students should be able to:
Explain the basic methodological approaches of palaeoproteomics
Understand the differences between methods applied to ancient proteins and select an appropriate approach
Prepare a sample for sex analysis from tooth enamel to final result and interpretation
Present the results of their project and provide feedback on other participants' projects
Syllabus
  • 1. Introduction to palaeoproteomics
  • 2. Protein preservation and modifications
  • 3. Sample selection and preparation for analysis
  • 4. Extraction and purification
  • 5. Mass spectrometry
  • 6. Basics of data analysis
  • 7. Authentication
  • 8. Practical session I - Sample preparation
  • 9. Practical session II - Extraction and purification
  • 10. Practical session III - Mass spectrometry
  • 11. Practical session IV - Data analysis
  • 12. Mini-conference
Literature
    recommended literature
  • TAUROZZI, Alberto J., et al. Deep-time phylogenetic inference by paleoproteomic analysis of dental enamel. Nature Protocols, 2024, 19.7: 2085-2116.
  • KOENIG, Claire, et al. Automated high-throughput biological sex identification from archeological human dental enamel using targeted proteomics. Journal of Proteome Research, 2024, 23.11: 5107-5121.
  • WARINNER, Christina; KORZOW RICHTER, Kristine; COLLINS, Matthew J. Paleoproteomics. Chemical reviews, 2022, 122.16: 13401-13446.
  • CHOCHOLOVÁ, Eva; Dana BURIÁNKOVÁ FIALOVÁ; Kateřina NOVOTNÁ and Eva DROZDOVÁ. Průvodce analýzami zubního kamene v archeologii (Guide to Dental Calculus Analysis in Archaeology). Acta Musei Nationalis Pragae – Historia. Praha: Národní muzeum, 2024, vol. 78, 1-2, p. 5-14. ISSN 2570-6845. Available from: https://dx.doi.org/10.37520/amnph.2024.002. URL info
  • CHOCHOLOVÁ, Eva; Pavel ROUDNICKÝ; David POTĚŠIL; Dana FIALOVÁ; Karolína KRYŠTOFOVÁ; Eva DROZDOVÁ and Zbyněk ZDRÁHAL. Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus. Journal of Proteome Research. American Chemical Society, 2023, vol. 22, No 10, p. 3311-3319. ISSN 1535-3893. Available from: https://dx.doi.org/10.1021/acs.jproteome.3c00370. URL info
Teaching methods
Presentations with discussions, practical laboratory classes, participation in the final mini-conference
Assessment methods
Mandatory attendance, final colloquium with presentation of own results and discussions on the topics.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught in blocks.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2025/Bi5137