PřF:Bi7665 Cell and Tissue Cultures - Course Information

Bi7665 Cell and Tissue Cultures

Extent and Intensity

1/0/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

Mgr. Jiří Pacherník, Ph.D. (lecturer)
Katarzyna Anna Radaszkiewicz, Ph.D., M.Sc. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)

Guaranteed by

prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Jiří Pacherník, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites (in Czech)

Bi2080 Histology and organology || Bi2120 Histology

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course the students are able to: Understand and explain principles of basic tissue culture techniques; Employ TC techniques in their own research; Routinely culture common cell lines; Characterize parameters of cell proliferation, differentiation, and apoptosis.

Learning outcomes

At the end of the course the students are able to: Understand and explain principles of basic tissue culture techniques; Employ TC techniques in their own research; Routinely culture common cell lines; Characterize parameters of cell proliferation, differentiation, and apoptosis.

Syllabus

• 1. Introduction to tissue culture; 2. basic prerequisites for tissue culture techniques; 3. invertebrates and poikilothermic animals; 4. homeothermic animals; 5. germ and stem cells; 6. embryonic stem and embryonal carcinoma cells; 7. differentiation of embryonic stem and embryonal carcinoma cells; 8. primo-culture; 9. adherent tumor cell lines; 10. suspension tumor cell lines; 11. tissue engineering and cell therapy; 12. culture of organs. Recommended literature Lesko, J. a kol.: Práce s tkanivovými kulturami, Bratislava, Vydavatelstvo slovenskej akademie ved 1975, s. 212. Alberts, B. a kol.: Základy buněčné biologie. Úvod do molekulární biologie buňky. Ústí nad Labem, Espero Publishing 1998, 630 s. Alberts, B. a kol.: Molecular biology of the cell. New York & London, Garland Publishing, Inc. 1994, 1294 s. Spector, D. L. a kol.: Cells. A laboratory manual. Volumes 1, 2, 3. New York, Cold Spring Harbor Laboratory Press 1998, 3 197 s. Cellis, J. E. a kol.: Cell Biology. A laboratory handbook. San Diego, London, Academic Press Inc. 1994, 1714 s. Sambrook, J. a kol.: Molecular Cloning. A laboratory manual. New York, Cold Spring Harbor Laboratory Press 1989, 1832 s. Ausubel, F. a kol.: Short Protocols in Molecular Biology. USA, Published by John Wiley & Sons Inc. 1995, 728 s.

Literature

• Basic methods in microscopy : protocols and concepts from cells : a laboratory manual. Edited by David L. Spector - Robert D. Goldman. 1st ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. ix, 382. ISBN 0879697512. 2006. info

Teaching methods

lectures

Assessment methods

Written test. The test consists of 10 questins for 3 points. Over 60% of correct answers is needed to pass (at least 18 points).

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!

Listed among pre-requisites of other courses

• Bi7665c Cell and tissue cultures - practical training
  Bi3030 && Bi4020 && Bi2080 && Bi7665  

• Enrolment Statistics (Autumn 2024, recent)
  
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