C3620 Biochemistry - laboratory course

Faculty of Science
Autumn 2025
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Lukáš Faltinek, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Lukáš Faltinek, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C3620/01: Wed 13:00–15:50 C15/212, V. Sedláček
C3620/02: Mon 14:00–16:50 C15/212, M. Pelcová
Prerequisites (in Czech)
NOW( C3580 Biochemistry )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 48 student(s).
Current registration and enrolment status: enrolled: 47/48, only registered: 0/48
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.
Learning outcomes
Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work
Syllabus
  • 01. Work safety rules. Organization of laboratory exercises. Preparation of dilute solution, pipetting, and photometry.
  • 02. Isolation, analysis, and determination of deoxyribonucleic acid (DNA).
  • 03. Qualitative and quantitative determination of amino acids.
  • 04. Quantitative determination of proteins.
  • 05. Qualitative and quantitative determination of carbohydrates.
  • 06. Isolation and analysis of lipids.
  • 07. Sucrase - immobilization, substrate specificity, activity determination.
  • 08. pH optimum of enzyme reaction.
  • 09. Enzyme kinetics.
  • 10. Analytical use of proteins.
  • 11. Biological electron transfer chains.
Literature
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald; Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Teaching methods
laboratory courses
Assessment methods
The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 60% of the questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
General note: Vyučují učitelé Ústavu biochemie.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2025/C3620