C6200 Biochemical Methods

Faculty of Science
Spring 2023
Extent and Intensity
4/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
doc. Mgr. Zdeněk Farka, Ph.D. (lecturer)
prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
Mgr. Marta Pelcová, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Zdeněk Farka, Ph.D.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
Tue 12:00–13:50 B11/205, Tue 16:00–17:50 B11/205
( c3181 Biochemistry I && C4182 Biochemistry II )|| C3580 Biochemistry
Basic knowledge of biochemistry, analytical and physical chemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The main goald of the subject are: understanding the basic methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of enzymes and biopolymers, chromatographic and electromigration methods, determination of purity and molecular properties of enzymes, spectroscopic, electrochemical, and physical methods of analysis in biochemistry.
Learning outcomes
At the end of this course, the students should not only theoretically understand but be able practically apply the methods of disintegration of animal, vegetal, and microbial tissues, methods of isolation, purification of proteins, nucleic acids, and other biopolymers, methods for determination of their purity and molecular properties, spectroscopic, electrochemical, and physical methods of analysis in biochemistry. They should be able to apply this knowledge in their scientific research working on bachelor, master and PhD theses and finally also during their following employment.
  • Block ZF (taught by Assoc. Prof. Zdeněk Farka)
    1. Electronic spectra of molecules, transitions, ground and excited state, influence of the environment, UV-VIS spectrophotometry, use to determine substances, use to study the structure of proteins.
    2. Atomic absorption and atomic emission spectrometry.
    3. Luminescence methods, quantum yield, influence of the environment
    4. Spectrofluorimetry, principle, use to determine substances, use to study protein conformation, fluorescence quenching, energy transfer, fluorescence, phosphorescence.
    5. IR spectroscopy and its use to study protein structure, Raman scattering and its use to study protein structure.
    6. Chiroptical methods and their principle, ORD and CD and their use to study protein conformation.
    7. Microscopy. Methods of optical microscopy, electron microscopy, scanning probe microscopy.
    8. Immunochemical methods. Preparation and use of antibodies in bioanalytical chemistry, formats of immunochemical assays.
    9. Biosensors. Biocatalytic and bioaffinity sensors, the most important types of transducers.
    10. Biochemical applications of nanoparticles. The most important types of nanoparticles, their preparation, characterization and use in bioanalytical chemistry.
  • Block MP (taught by Dr. Marta Pelcová)
    1. The strategy of treatement of biological material.
    2. Disintegration, centrifugation, sedimentation analysis.
    3. Phase separation, precipitation, extraction. membrane separation, Concentration and lyophilisation, water treatment.
    4. Chromatographic methods, principles (adsorption, ion-exchange), chromatofocusation.
    5. Reversed phase chromatography, ion pair chromatography, hydrophobic, GPC, affinity, gas chromatography.
    6. Electromigration methods, principles, zone electrophoresis
    7. IEF, isotachophoresis, blotting.
    8. Methods of determination of mol. mass and shape of molecules.
    9. Mass spectrometry.
    recommended literature
  • KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987. 430 s. URL info
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986. 199 s. info
  • KALOUS, Vítěz and Zdeněk PAVLÍČEK. Biofyzikální chemie. 1. vyd. Praha: Nakladatelství technické literatury, 1980. 349 s. info
Teaching methods
Lectures with a demonstration of the taught topic by means of demonstration videos and programs.
Assessment methods
Basic lecture given by Assoc. Prof. Zdeněk Farka and Dr. Marta Pelcová. The exam is in the written form. Examiner Assoc. Prof. Zdeněk Farka.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022.
  • Enrolment Statistics (recent)
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