C6200 Biochemical Methods

Faculty of Science
Spring 2024
Extent and Intensity
4/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Zdeněk Farka, Ph.D. (lecturer)
Mgr. Marta Pelcová, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Zdeněk Farka, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Tue 12:00–13:50 B11/205, Tue 16:00–17:50 B11/205
Prerequisites
( c3181 Biochemistry I && C4182 Biochemistry II )|| C3580 Biochemistry
Basic knowledge of biochemistry, analytical and physical chemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The main goals of the subject are: understanding the basic methods of disintegration of animal, plant, and microbial tissues, methods of isolation and purification of enzymes and biopolymers, chromatographic and electromigration methods, determination of purity and molecular properties of enzymes, spectroscopic, electrochemical, and physical methods of biochemical analysis.
Learning outcomes
At the end of this course, the students will be able to theoretically understand and practically apply the methods of disintegration of animal, plant, and microbial tissues, methods of isolation and purification of proteins, nucleic acids, and other biopolymers, methods for the determination of their purity and molecular properties, spectroscopic, electrochemical, and physical methods of analysis in biochemistry. They will able to apply this knowledge in their scientific research, working on bachelor, master, and PhD theses and finally also during their following employment.
Syllabus
  • Block ZF (taught by Assoc. Prof. Zdeněk Farka)
    1. Electronic spectra of molecules, transitions, ground and excited state, influence of the environment, UV-Vis spectroscopy, use to determine substances and study the structure of proteins.
    2. Chiroptical methods and their principle. ORD and CD and their use to study protein conformation.
    3. IR spectroscopy and its use to study protein structure, Raman scattering and its use to study protein structure.
    4. Atomic absorption and atomic emission spectroscopy.
    5. Luminescence methods. Quantum yield, influence of the environment, use to determine substances and study protein conformation, fluorescence quenching, energy transfer.
    6. Microscopy. Methods of optical microscopy, electron microscopy, and scanning probe microscopy.
    7. Immunochemical methods. Preparation and use of antibodies in bioanalytical chemistry, formats of immunochemical assays.
    8. Biosensors. Biocatalytic and bioaffinity sensors, the most important types of transducers.
    9. Biochemical applications of nanoparticles. The most important types of nanoparticles, their preparation, characterization, and use in bioanalytical chemistry.
  • Block MP (taught by Dr. Marta Pelcová)
    1. The strategy of treatement of biological materials.
    2. Disintegration, centrifugation, sedimentation analysis.
    3. Phase separation, precipitation, extraction. Membrane separation, concentration and lyophilisation, water treatment.
    4. Chromatographic methods, principles (adsorption, ion-exchange), chromatofocusing.
    5. Reversed phase chromatography, ion pair chromatography, hydrophobic, GPC, affinity, gas chromatography.
    6. Electromigration methods, principles, zone electrophoresis.
    7. IEF, isotachophoresis, blotting.
    8. Methods of determination of molar mass and shape of molecules.
    9. Mass spectrometry.
Literature
  • HOLTZHAUER, Martin. Basic methods for the biochemical lab. 1st english ed. Berlin: Springer. xviii, 251. ISBN 3540327851. 2006. URL info
  • PINGOUD, Alfred. Biochemical methods : a concise guide for students and researchers. Weinheim: Wiley-VCH. xiii, 360. ISBN 3527302999. 2002. info
Teaching methods
Lectures with a demonstration of the taught topics by means of demonstration videos and programs.
Assessment methods
Lecture given by Assoc. Prof. Zdeněk Farka and Dr. Marta Pelcová. The exam is in the written form. Examiner Assoc. Prof. Zdeněk Farka.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023.
  • Enrolment Statistics (recent)
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