C4830 Instrumental Methods in Biochemistry

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
C3181 Biochemistry I || C3580 Biochemistry
Basic knowledge of general biochemistry in range of the lectures Biochemistry I (C3181) or Biochemistry(C3580).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The students should have the basic knowledge of the separation methods in biochemistry or molecular biology at the end of the course. Contents of the first part include the basic manipulation with biological samples - extraction, clarification, precipitation, ultrafiltration and lyophilisation. Second part is oriented onto chromatography methods. Last part is dedicated in electromigration methods.
Learning outcomes
At the end of this course the students should not only theoretically understand but be able practically apply the methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of proteins, nucleic acids and other biopolymers, methods for determination their purity and molecular properties. They should be able to apply these knowledges in their scientific research working on bachelor, master and PhD theses and finally during their following employment as well.
  • 1.Introduction. Basic Preparation. Purification Strategy. 2.Clarification and Extraction. Centrifugation. 3.Phase Separation. Precipitation and Selective Denaturation. Membrane Separation. 4.Ultrafiltration and Lyophilisation. Laboratory Water Preparation. 5.Chromatography. Basic Principles. 6.Adsorption and Partition Chromatography. 7.Ion-exchange Chromatography. Chromatofocusing. 8.Reverse Phase and Ion Pair Chromatography. Hydrophobic Chromatography 9.Gel Permeation Chromatography. 10.Affinity Chromatography. 11.Gas Chromatography. 12.Electromigration Metods. Basic Principles. Free and Zone Electrophoresis. 13.Isoelectric Focusing. 14.Isotachophoresis.
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986. 199 s. info
  • SNYDER, Lloyd R., Joseph Jack KIRKLAND and Joseph L. GLAJCH. Practical HPLC method development [Snyder, 1997]. 2nd ed. New York: John Wiley & Sons, 1997. xxvi, 765. ISBN 0-471-00703-X. info
Teaching methods
Lectures with the demonstrations of the taught topic by means of educational videos and programs that are available for the students on web page of this course.
Assessment methods
The final exam is in the form of written test, which contains questions from the sample extraction, centrifugation, electromigration and chromatographic methods. The time for the test is one hour, the grades are A to F on the basis of each question classification.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught once in two years.
Information on the per-term frequency of the course: (sudé roky).
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2019.
  • Enrolment Statistics (recent)
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