C4830 Instrumental Methods in Biochemistry

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: k (colloquium).
Teacher(s)
prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Prerequisites
C3181 Biochemistry I || C3580 Biochemistry
Basic knowledges from general biochemistry in range of the lectures Biochemistry I (C3181) or Biochemistry(C3580).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The objective of the lecture is to get to students the basic knowledges from separation methods in biochemistry or molecular biology. Contents of the first part includes the basic manipulation with biological samples - extraction, clarification, precipitation, ultrafiltration and lyophilisation. Second part is orientied onto chromatography methods. Last part is dedicated in electromigration methods.
Syllabus
  • 1.Introduction. Basic Preparation. Purification Strategy. 2.Clarification and Extraction. Centrifugation. 3.Phase Separation. Precipitation and Selective Denaturation. Membrane Separation. 4.Ultrafiltration and Lyophilisation. Laboratory Water Preparation. 5.Chromatography. Basic Principles. 6.Adsorption and Partition Chromatography. 7.Ion-exchange Chromatography. Chromatofocusing. 8.Reverse Phase and Ion Pair Chromatography. Hydrophobic Chromatography 9.Gel Permeation Chromatography. 10.Affinity Chromatography. 11.Gas Chromatography. 12.Electromigration Metods. Basic Principles. Free and Zone Electrophoresis. 13.Izoelectric Focusing. 14.Isotachophoresis.
Literature
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986. 199 s. info
  • SNYDER, Lloyd R., Joseph Jack KIRKLAND and Joseph L. GLAJCH. Practical HPLC method development [Snyder, 1997]. 2nd ed. New York: John Wiley & Sons, 1997. xxvi, 765. ISBN 0-471-00703-X. info
Assessment methods (in Czech)
Zkouška probíhá písemnou formou, studenti přitom dostávají test, který zahrnuje otázky z metod extrakce a přípravy vzorků, centrifugačních, chromatografických a elektronmigračních metod. Doba testu je 1 hodina. Klasifikace je "uspěl nebo "neuspěl".
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught once in two years.
Information on the per-term frequency of the course: (sudé roky).
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://orion.chemi.muni.cz/biochem/student.htm
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2019, Spring 2021.