PřF:C6230 Clinical Biochem.-lab.course - Course Information
C6230 Clinical Biochemistry - laboratory course
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 0/4/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor) - Guaranteed by
- doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: RNDr. Jitka Kašparovská, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine - Timetable of Seminar Groups
- C6230/01: Tue 9:00–12:50 C15/212, J. Tomandl
C6230/02: Tue 13:00–16:50 C15/212, J. Tomandl - Prerequisites (in Czech)
- C6221 Clinical Biochemistry || NOW ( C6221 Clinical Biochemistry )
Předpokladem zápisu je současné absolvování předmětu klinická biochemie (C6220). K zápisu je potřeba požádat o výjimku-souhlas učitele. Souhlas bude udělován v pořadí: Aplikovaná biochemie (předmět je povinný), Biochemie (předmět je povinně volitelný), ostatní. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 48 student(s).
Current registration and enrolment status: enrolled: 7/48, only registered: 41/48, only registered with preference (fields directly associated with the programme): 37/48 - fields of study / plans the course is directly associated with
- Analytical biochemistry (programme PřF, N-BIC)
- Applied biochemistry (programme PřF, B-BIC)
- Biochemistry (programme PřF, B-BIC)
- Course objectives
- The aim of the course is to acquaint students with the basic methodological principles and procedures used in the determination of analytes and enzyme activity in body fluids as well as basic ways of interpretion of laboratory results.
- Learning outcomes
- Through laboratory experiences the student will develop the necessary skills to perform analytical procedures and evaluate laboratory data. The exercises familiarize the student with the performance of simple laboratory tests common in health care centers.
At the end of the course, students should be able to:
- understand basic principles of basic analytical parameters determination in blood serum and urine;
- determine basic biochemical parameters in serum and urine;
- interpret measured results in the context of potential health/disease.
- understand basic principles of basic analytical parameters determination in blood serum and urine;
- Syllabus
- Principles of laboratory biosafety and biosecurity. Collection and processing of blood/urine. Interpretation of results. Biochemical analyzers.
- Determination of triglycerides. Determination of cholesterol (total, HDL) in serum and blood. Calculation of LDL-cholesterol and derived parameters.
- Determination of glucose in blood and serum. Oral glucose tolerance test (OGTT). Determination of glycated hemoglobin. Determination glucosuria and ketonuria.
- Determination of total protein in serum. Electrophoresis of serum proteins. Determination of serum albumin. Determination of serum proteins by ELISA. Determination of cardiomarkers by POCT. Estimation of C-reactive protein.
- Determination of catalytic concentration of serum enzymes (ALT / AST, ALP isoenzymes, total CK, CK-MB) by kinetic and constant time methods, influence of temperature. Determination of the mass concentration of CK-MBmass.
- Determination of nitrogen metabolites in serum/urine (total bilirubin, conjugated, uric acid, urea). Approximate evaluation of nitrogen balance.
- Analysis of urinary proteins (proteinurias, albuminuria, Bence-Jones protein). Chemical dipstick testing.
- Objective and physical examination urine. Determination of urine osmolality. Determination of serum/urine creatinine. Creatinine clearance and estimation of glomerular filtration rate. Fractional excretion and reabsorption of water.
- Indicative chemical analysis of urinary stones.
- Laboratory tests of saliva (rate of salivation, buffering capacity, lactate) Examination of gastrointestinal tract Laboratory examination of stomach (gastric acid output, confirmation of Helicobacter pylori by breath and urease tests). Laboratory test in pancreatic disease (α-amylase, secretin-CCK and NBT-PABA and MTG breath tests). Test of occult bleeding in GIT.
- Solution of model cases.
- Literature
- required literature
- Podklady pro jednotlivá cvičení v Interaktivní osnově předmětu C6230 v IS MU
- recommended literature
- Rajdl. D. a kol. Clinical biochemistry (e-book, formats: .pdf, .epub, .mobi) 1st ed. Prague, Karolinum Press, 2016, 426 s. ISBN: 978-80-246-3497-5. Download: https://karolinum.cz/en/books/rajdl-clinical-biochemistry-17096
- RACEK, Jaroslav and Daniel RAJDL. Klinická biochemie. Třetí, přepracované a ro. Praha: Galén, 2021, 454 stran. ISBN 9788074925450. info
- Teaching methods
- Laboratory courses
- Assessment methods
- Credit. Conditions for granting credit:
1) completion of all exercises, incl. submission of lab reports, 2) completion of progress tests.
Failure to meet the requirements of the progess tests requires a remedial test which may be repeated once.
Detailed conditions are published in IS MUNI (Study materials – Course-related instructions). - Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/C6230