C6230 Clinical Biochemistry - laboratory course

Faculty of Science
Autumn 2024
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Taught in person.
Teacher(s)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: RNDr. Jitka Kašparovská, Ph.D.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
C6221 Clinical Biochemistry || NOW ( C6221 Clinical Biochemistry )
Předpokladem zápisu je současné absolvování předmětu klinická biochemie (C6220). K zápisu je potřeba požádat o výjimku-souhlas učitele. Souhlas bude udělován v pořadí: Aplikovaná biochemie (předmět je povinný), Biochemie (předmět je povinně volitelný), ostatní.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 50 student(s).
Current registration and enrolment status: enrolled: 0/50, only registered: 0/50, only registered with preference (fields directly associated with the programme): 0/50
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the basic methodological principles and procedures used in the determination of analytes and enzyme activity in body fluids as well as basic ways of interpretion of laboratory results.
Learning outcomes
Through laboratory experiences the student will develop the necessary skills to perform analytical procedures and evaluate laboratory data. The exercises familiarize the student with the performance of simple laboratory tests common in health care centers.
At the end of the course, students should be able to:
  • understand basic principles of basic analytical parameters determination in blood serum and urine;
  • determine basic biochemical parameters in serum and urine;
  • interpret measured results in the context of potential health/disease.
    • Syllabus
    • Principles of laboratory biosafety and biosecurity. Collection and processing of blood/urine. Interpretation of results. Biochemical analyzers.
    • Determination of triglycerides. Determination of cholesterol (total, HDL) in serum and blood. Calculation of LDL-cholesterol and derived parameters.
    • Determination of glucose in blood and serum. Oral glucose tolerance test (OGTT). Determination of glycated hemoglobin. Determination glucosuria and ketonuria.
    • Determination of total protein in serum. Electrophoresis of serum proteins. Determination of serum albumin. Determination of serum proteins by ELISA. Determination of cardiomarkers by POCT. Estimation of C-reactive protein.
    • Determination of catalytic concentration of serum enzymes (ALT / AST, ALP isoenzymes, total CK, CK-MB) by kinetic and constant time methods, influence of temperature. Determination of the mass concentration of CK-MBmass.
    • Determination of nitrogen metabolites in serum/urine (total bilirubin, conjugated, uric acid, urea). Approximate evaluation of nitrogen balance.
    • Analysis of urinary proteins (proteinurias, albuminuria, Bence-Jones protein). Chemical dipstick testing.
    • Objective and physical examination urine. Determination of urine osmolality. Determination of serum/urine creatinine. Creatinine clearance and estimation of glomerular filtration rate. Fractional excretion and reabsorption of water.
    • Indicative chemical analysis of urinary stones.
    • Laboratory tests of saliva (rate of salivation, buffering capacity, lactate) Examination of gastrointestinal tract Laboratory examination of stomach (gastric acid output, confirmation of Helicobacter pylori by breath and urease tests). Laboratory test in pancreatic disease (α-amylase, secretin-CCK and NBT-PABA and MTG breath tests). Test of occult bleeding in GIT.
    • Solution of model cases.
    Literature
      required literature
    • Podklady pro jednotlivá cvičení v Interaktivní osnově předmětu C6230 v IS MU
      recommended literature
    • Rajdl. D. a kol. Clinical biochemistry (e-book, formats: .pdf, .epub, .mobi) 1st ed. Prague, Karolinum Press, 2016, 426 s. ISBN: 978-80-246-3497-5. Download: https://karolinum.cz/en/books/rajdl-clinical-biochemistry-17096
    • RACEK, Jaroslav and Daniel RAJDL. Klinická biochemie. Třetí, přepracované a ro. Praha: Galén, 2021, 454 stran. ISBN 9788074925450. info
    Teaching methods
    Laboratory courses
    Assessment methods
    Credit. Conditions for granting credit:
    1) completion of all exercises, incl. submission of lab reports, 2) completion of progress tests.
    Failure to meet the requirements of the progess tests requires a remedial test which may be repeated once.
    Detailed conditions are published in IS MUNI (Study materials – Course-related instructions).
    Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    The course is taught: every week.
    The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
    • Enrolment Statistics (Autumn 2024, recent)
    • Permalink: https://is.muni.cz/course/sci/autumn2024/C6230