C7301 Genomics - practice

Faculty of Science
Autumn 2025
Extent and Intensity
0/0/3. 3 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: k (colloquium). Other types of completion: z (credit).
In-person direct teaching
Teacher(s)
doc. RNDr. Jan Hejátko, Ph.D. (seminar tutor)
Ing. Šárka Michlíčková (seminar tutor)
Mgr. Jiří Rudolf (seminar tutor)
Mgr. Anna Rudolfová (seminar tutor)
RNDr. Jan Skalák, Ph.D. (seminar tutor)
Mgr. Lucia Tomovičová (seminar tutor)
Guaranteed by
doc. RNDr. Jan Hejátko, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. RNDr. Jan Hejátko, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
NOW( CG020 Genomics )
Completion of basic courses of biochemistry and/or molecular biology and genetics is a prerequisite for subscription to the course.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 36 student(s).
Current registration and enrolment status: enrolled: 12/36, only registered: 0/36, only registered with preference (fields directly associated with the programme): 0/36
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
Students will gain practical experience with basic methods of functional genomics, i.e. basic approaches for in silico DNA sequence analysis, gene expression analysis using transcriptional fusion and automated microscopy, quantification of protein-protein interactions in vivo using FlIM/FRET method, identification of insertion mutants using PCR and quantification of gene expression using RT qPCR. The work is carried out in groups of 2, so everyone can practically try out an independent approach to both experiment planning and execution.
Learning outcomes
At the end of the course, the students will get the overview of basic approaches of the functional genomics. Students will be able to aply them for solution of problems during their own scientific praxis.
Syllabus
  • Working with molecular biology databases
  • Analysis of gene expression using transcription fusion
  • Quantification of protein-protein interactions in vivo via FLIM/FRET
  • Isolation of plant genomic DNA
  • Identification of insertion mutants using PCR
  • Isolation of RNA
  • Quantification of gene expression using RT qPCR
  • Basics of automated and confocal microscopy
Literature
  • Functional genomics : methods and protocols. Edited by Michael J. Brownstein - Arkady B. Khodursky. Totowa, N.J.: Humana Press, 2003, xii, 258. ISBN 1588292916. info
  • Plant functional genomics. Edited by Erich Grotewold. Totowa, N.J.: Humana Press, 2003, xv, 449 s. ISBN 1588291456. info
Teaching methods
Practical solution of problems demonstrating utilizing of basic instruments of the functional genomics.
Assessment methods
The course will end up by colloquium. The prerequisite for successful completion of the course is timely submission of required protocols and participation in the final interview.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught in blocks.
Information on course enrolment limitations: pro zápis do cvičení je nutné absolvování teoretické přednášky Bi7201
Teacher's information
The course will be taught in a week block from Dec 15 until Dec 19 2025.
The course is also listed under the following terms Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2025/C7301