PřF:C7195 Advance biochem. lab. course - Course Information
C7195 Advance laboratory course of biochemistryFaculty of Science
- Extent and Intensity
- 0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
- doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
- Guaranteed by
- doc. Mgr. Tomáš Kašparovský, Ph.D.
Department of Biochemistry - Chemistry Section - Faculty of Science
Contact Person: doc. Mgr. Tomáš Kašparovský, Ph.D.
- C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody
- Course Enrolment Limitations
- The course is offered to students of any study field.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24
- Course objectives
- At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows : separation and isolation of bimolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinitive chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.
- Cloning of gen FerB to plasmid pGEM T-Easy Identification of Armillara sp. in soil by nested PCR-RFLP analysis Determination of changes in expression of gen for PR1a protein Study of changes in tobacco proteome during defense reaction by 2-D electrophoresis Identification of glycoprotein’s in blood serum by SDS-PAGE a Western blot. Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Isolation of enzyme FerB from Paracoccus denitrificans cells Study interaction of ligand-protein
- Návody ke cvičením - viz Studijní materiály
- Teaching methods
- Laboratory course
- Assessment methods
- Credit course
- Language of instruction
- Further Comments
- Study Materials
The course is taught annually.
The course is taught: in blocks.