C7195 Advance laboratory course of biochemistry

Faculty of Science
Autumn 2019
Extent and Intensity
0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
Mgr. David Novák (assistant)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry - Chemistry Section - Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
Prerequisites
C4220 Biochemie - laboratorní cvičení C6200 Biochemické metody
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should understand the recently teachings of biochemistry which can be summarized follows: separation and isolation of biomolecules (proteins, nucleic acids, low molecular compounds), purification biomolecules (affinity chromatography), measuring interaction between biomolecules, methods for enzymes activity determination, immunochemical methods, work with cell cultures and modern diagnostics methods.
Learning outcomes
Student will be able to: - Perform PCR-RFLP analysis - Isolate RNA from biological material - Perform reverse transcription - Perform qPCR and evaluate data - Perform 2D electrophoresis - Perform native electrophoresis - Expressed recombinant proteins in E. coli and isolate them - Conduct critical evaluation of obtained data
Syllabus
  • Identification of Armillara sp. in soil by nested PCR-RFLP analysis The role polymorphism in ADH gene for its activity Cloning of PCR product into plasmid Study of Paracoccus denitrificans proteome changes in alteration from anaerobic conditions to aerobic conditions by 2D-electrophoresis Detection of isoenzymes for ascorbate peroxidase and peroxidase by native PAGE Determination of changes in PR genes expression in tobacco plants Expression and purification of recombinant proteins Use of bioluminescence in vaccine preparation
Literature
  • Návody ke cvičením - viz Studijní materiály
Teaching methods
Laboratory course
Assessment methods
Credit course
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2020, autumn 2021.
  • Enrolment Statistics (Autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2019/C7195