Bi6270c Cytogenetics - practice

Faculty of Science
Spring 2011
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Vladimíra Vallová, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Timetable
Mon 9:00–12:50 C13/332
  • Timetable of Seminar Groups:
Bi6270c/A: No timetable has been entered into IS. V. Vallová
Bi6270c/B: No timetable has been entered into IS.
Bi6270c/C: No timetable has been entered into IS.
Prerequisites
Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW( Bi4020 Molecular biology )) && NOW( Bi6270 Cytogenetics )
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 45 student(s).
Current registration and enrolment status: enrolled: 0/45, only registered: 0/45
fields of study / plans the course is directly associated with
Course objectives
Cytogenetics is one of the oldest branches of genetics. The course provides undergraduate students the general introduction to cytogenetics. Main objectives can be summarized as follows: to understand main aspect of chromosome biology and to learn modern cytogenetic techniques and methods. At the end of this course, students will be able to prepare chromosomal samples using standard chromosome banding methods used in conventionl cytogenetics. They will also be able to interpret human karyotype. They will understand the principles of FISH technique and be able to prepare samples for it, as well as to analyse the results obtained by digital image analysis. They will be able to explain the basic principles of other molecular cytogenetics methods (mFISH, SKY, CGH, arrayCGH) and their applications in clinical genetics and oncology.
Syllabus
  • 1.Technical equipment of cytogenetic laboratory. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. 2. Basic techniques of cytogenetic preparation making. Plant cytogenetics. Pretreatment, fixation, maceration, Cytochemical and histochemical staining methods, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Human cytogenetics. Chromosome identification, karyotyp, idiogram. Chromosome banding methods. Q, G, C, R, N-banding. Chromosomal aberrations. Cytogenetic abnormalities in hematological malignancies and solid tumors. An International System for Human Cytogenetic Nomenclature (ISCN). 4. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. 5. Application of computer image analysis in human cytogenetics.
Literature
  • Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
  • MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
  • Chromosome analysis protocols. Edited by John R. Gosden. Totowa: Humana Press, 1994, xi, 508 s. ISBN 0-89603-243-4. info
Teaching methods
Laboratory courses. Powerpoint presentations are used together with practical exercises and display of specific laboratory techniques directly in clinical laboratories.
Assessment methods
Credit for participation in the laboratory courses.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2011/Bi6270c